Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/26290
Title: Distinct patterns of cellular immune response elicited by influenza non-adjuvanted and AS03-adjuvanted monovalent H1N1(pdm09) vaccine
Authors: Silva, Sarah Giarola da
Reis, Jordana Graziela Alves Coelho dos
Mourao, Marina Moraes
Azevedo, Ana Carolina Campi
Silva, Erick E. Nakagaki
Silva, Maria Luiza
Martins, Marina Angela
Cassette, Amanda Cardoso de Oliveira Silveira
Batista, Maurício Azevedo
Pascoal, Vanessa Peruhype Magalhaes
Antonelli, Lis Ribeiro do Valle
Ribeiro, Jose Geraldo Leite
Santos, Silvana Maria Eloi
Machado, Alexandre Vieira
Carvalho, Andrea Teixeira de
Martins Filho, Olindo Assis
Araújo, Marcio Sobreira Silva
Affilliation: Fundaçao Oswaldo Cruz. Instituto Rene Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brazil
Fundaçao Oswaldo Cruz. Instituto Rene Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brazil
Fundaçao Oswaldo Cruz. Instituto Rene Rachou. Grupo Helmintologia e Malacologia Medica. Belo Horizonte, MG, Brazil
Fundaçao Oswaldo Cruz. Instituto Rene Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brazil
Universidade Federal de Minas Gerais. Faculdade de Medicina. Belo Horizonte, MG, Brazil
Fundaçao Oswaldo Cruz. Instituto Rene Rachou. Grupo Helmintologia e Malacologia Medica. Belo Horizonte, MG, Brazil
Fundaçao Oswaldo Cruz. Instituto Rene Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brazil
Fundaçao Oswaldo Cruz. Instituto Rene Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brazil
Fundaçao Oswaldo Cruz. Instituto Rene Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brazil
Fundaçao Oswaldo Cruz. Instituto Rene Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brazil
Fundaçao Oswaldo Cruz. Instituto Rene Rachou. Biologia e Imunologia Parasitaria. Belo Horizonte, MG, Brazil
Secretaria Estadual de Saúde de Minas Gerais. Belo Horizonte, MG, Brazil
Fundaçao Oswaldo Cruz. Instituto Rene Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brazil/Universidade Federal de Minas Gerais. Faculdade de Medicina. Belo Horizonte, MG, Brazil
Fundaçao Oswaldo Cruz. Instituto Rene Rachou. Imunologia de Doenças Virais. Belo Horizonte, MG, Brazil
Fundaçao Oswaldo Cruz. Instituto Rene Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brazil
Fundaçao Oswaldo Cruz. Instituto Rene Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brazil
Fundaçao Oswaldo Cruz. Instituto Rene Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brazil
Abstract: The study aimed at identifying biomarkers of immune response elicited by non-adjuvanted-(NAV) and adjuvanted-(AV) H1N1(pdm09) vaccines. The results showed that despite both vaccines elicited similar levels of anti-H1N1 antibodies at day30 after vaccination, higher reactivity was observed in AV at day180. While AV induced early changes in cell-surface molecules on monocytes, CD4þ, CD8þ T-cells and B-cells, NAV triggered minor changes, starting later on at day3. Furthermore, AV induced a late and persistent increase in TLR gene expression after day3, except for tlr4, while NAV displayed earlier but transient tlr3/ 4/7/9 up-regulation. Contrasting with NAV, prominent chemokine gene expression (cxcl8,cxcl9,ccl5) and a broad spectrum up-regulation of plasmatic biomarkers (CXCL8,IL-6,IL-1b,IL-12,IL-10) was evident in AV, which showed a major involvement of TNF and IL-10. Similarly, AV induced a robust IL-10-modulated proinflammatory storm, with early and persistent involvement of TNF-a/IL-12/IFN-g axis derived from NK-cells, CD4þ and CD8þ T-cells along with promiscuous production of IL-4/IL-5/IL-13. Conversely, NAV promotes a concise and restricted intracytoplasmic chemokine/cytokine response, essentially mediated by TNF-a and IL-4, with late IL-10 production by CD8þ T-cells. Systems biology approach underscored that AV guided the formation of an imbricate network characterized by a progressive increase in the number of neighborhood connections amongst innate and adaptive immunity. In AV, the early cross-talk between innate and adaptive immunity, followed by the triad NK/CD4þ/CD8þ T-cells at day3, sponsored a later/robust biomarker network. These findings indicate the relevance of adjuvanted vaccination to orchestrate broad, balanced and multifactorial cellular immune events that lead ultimately to a stronger H1N1 humoral immunity.
Keywords: H1N1
vaccine
AS03 adjuvant
Immune response
Biomarker
signature
keywords: vacina
Issue Date: 2017
Publisher: Elsevier Masson
Citation: SILVA, Sarah Giarola da et al. Distinct patterns of cellular immune response elicited by influenza non-adjuvanted and AS03-adjuvanted monovalent H1N1(pdm09) vaccine. Antiviral Res., v. 144, p. 70-82, 2017.
DOI: 10.1016/j.antiviral.2017.05.009
ISSN: 0166-3542
Copyright: restricted access
Appears in Collections:MG - IRR - Artigos de Periódicos

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