Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/26532
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dc.contributor.authorAlessio, Glaucia Diniz
dc.contributor.authorAraujo, Fernanda Fortes de
dc.contributor.authorCortes, Denise Fonseca
dc.contributor.authorSales Junior, Policarpo Ademar
dc.contributor.authorLima, Daniela Cristina
dc.contributor.authorGomes, Matheus de Souza
dc.contributor.authorAmaral, Laurence Rodrigues do
dc.contributor.authorXavier, Marcelo Antonio Pascoal
dc.contributor.authorCarvalho, Andrea Teixeira de
dc.contributor.authorMartins Filho, Olindo Assis
dc.contributor.authorLana, Marta de
dc.date.accessioned2018-05-21T18:05:11Z
dc.date.available2018-05-21T18:05:11Z
dc.date.issued2017
dc.identifier.citationALESSIO, Glaucia Diniz et al. Performance of TcI/TcVI/TcII Chagas-Flow ATE-IgG2a for universal and genotype-specific serodiagnosis of Trypanosoma cruzi infection. PLoS Neglected Tropical Diseases, v. 11, p. e0005444-24, 2017
dc.identifier.issn1935-2727
dc.identifier.urihttps://www.arca.fiocruz.br/handle/icict/26532
dc.language.isoeng
dc.publisherPublic Library of Science
dc.rightsopen access
dc.subject.otherTrypanosoma cruzi
dc.subject.otherDoença de Chagas
dc.titlePerformance of TcI/TcVI/TcII Chagas-Flow ATE-IgG2a for universal and genotype-specific serodiagnosis of Trypanosoma cruzi infection
dc.typeArticle
dc.identifier.doi10.1371/journal.pntd.0005444
dc.description.abstractenDistinct Trypanosoma cruzi genotypes have been considered relevant for patient management and therapeutic response of Chagas disease. However, typing strategies for genotype- specific serodiagnosis of Chagas disease are still unavailable and requires standardization for practical application. In this study, an innovative TcI/TcVI/TcII Chagas Flow ATE-IgG2a technique was developed with applicability for universal and genotypespecific diagnosis of T. cruzi infection. For this purpose, the reactivity of serum samples (percentage of positive fluorescent parasites-PPFP) obtained from mice chronically infected with TcI/Colombiana, TcVI/CL or TcII/Y strain as well as non-infected controls were determined using amastigote-AMA, trypomastigote-TRYPO and epimastigote-EPI in parallel batches of TcI, TcVI and TcII target antigens. Data demonstrated that ªα-TcII-TRYPO/ 1:500, cut-off/PPFP = 20%º presented an excellent performance for universal diagnosis of T. cruzi infection (AUC = 1.0, Se and Sp = 100%). The combined set of attributes ªα-TcITRYPO/ 1:4,000, cut-off/PPFP = 50%º, ªα-TcII-AMA/1:1,000, cut-off/PPFP = 40%º and ªα- TcVI-EPI/1:1,000, cut-off/PPFP = 45%º showed good performance to segregate infections with TcI/Colombiana, TcVI/CL or TcII/Y strain. Overall, hosts infected with TcI/Colombiana and TcII/Y strains displayed opposite patterns of reactivity with ªα-TcI TRYPOº and ªα-TcII AMAº. Hosts infected with TcVI/CL strain showed a typical interweaved distribution pattern. The method presented a good performance for genotype-specific diagnosis, with global accuracy of 69% when the population/prototype scenario include TcI, TcVI and TcII infections and 94% when comprise only TcI and TcII infections. This study also proposes a receiver operating reactivity panel, providing a feasible tool to classify serum samples from hosts infected with distinct T. cruzi genotypes, supporting the potential of this method for universal and genotype-specific diagnosis of T. cruzi infection.
dc.creator.affilliationUniversidade Federal de Ouro Preto. Instituto de Ciências Exatas e Biologicas. Nucleo de Pesquisas em Ciências Biologicas. Laboratorio de Doença de Chagas. Ouro Preto, MG, Brazil
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Rene Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brazil
dc.creator.affilliationUniversidade Federal de Ouro Preto. Instituto de Ciências Exatas e Biologicas. Nucleo de Pesquisas em Ciências Biologicas. Laboratorio de Doença de Chagas. Ouro Preto, MG, Brazil
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Rene Rachou. Grupo de Genomica Funcional e Proteomica de Leishmania spp e Trypanosoma cruzi. Belo Horizonte, MG, Brazil
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Rene Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brazil
dc.creator.affilliationUniversidade Federal de Uberlandia. Laboratorio de Bioinformatica e Analises Moleculares. Patos de Minas, MG, Brazil
dc.creator.affilliationUniversidade Federal de Uberlandia. Laboratorio de Bioinformatica e Analises Moleculares. Patos de Minas, MG, Brazil
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Rene Rachou. Grupo de Pesquisas Clınicas e Polıticas Publicas em Doenças Infecciosas e Parasitarias. Belo Horizonte, MG, Brazil
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Rene Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brazil
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Rene Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brazil
dc.creator.affilliationUniversidade Federal de Ouro Preto. Instituto de Ciências Exatas e Biologicas. Nucleo de Pesquisas em Ciências Biologicas. Laboratorio de Doença de Chagas. Ouro Preto, MG, Brazil
dc.subject.enTrypanosoma cruzi
dc.subject.enChagas disease
Appears in Collections:MG - IRR - Artigos de Periódicos

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