| Author | Andrade, Ana Cláudia dos Santos Pereira | |
| Author | Rodrigues, Rodrigo Araújo Lima | |
| Author | Oliveira, Graziele Pereira | |
| Author | Andrade, Kétyllen Reis | |
| Author | Bonjardim, Cláudio Antônio | |
| Author | Scola, Bernard La | |
| Author | Kroon, Erna Geessien | |
| Author | Abrahão, Jônatas Santos | |
| Access date | 2018-06-14T17:23:34Z | |
| Available date | 2018-06-14T17:23:34Z | |
| Document date | 2017 | |
| Citation | ANDRADE, Ana Cláudia dos Santos Pereira et al. Filling Knowledge Gaps for Mimivirus Entry, Uncoating, and Morphogenesis. J Virol. , v. 91, n. 22, e01335-17, 2017. | pt_BR |
| ISSN | 0022-538X | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/26897 | |
| Language | eng | pt_BR |
| Publisher | American Society for Microbiology | pt_BR |
| Rights | restricted access | pt_BR |
| Subject in Portuguese | mimivirus | pt_BR |
| Subject in Portuguese | microscopia eletronica | pt_BR |
| Subject in Portuguese | ciclo de replicação | pt_BR |
| Subject in Portuguese | fagocitose | pt_BR |
| Subject in Portuguese | área de aquisição de fibrilas | pt_BR |
| Title | Filling Knowledge Gaps for Mimivirus Entry, Uncoating, and Morphogenesis | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1128/JVI.01335-17 | |
| Abstract | Since the discovery of mimivirus, its unusual structural and genomic features have raised great interest in the study of its biology; however, many aspects concerning its replication cycle remain uncertain. In this study, extensive analyses of electron microscope images, as well as biological assay results, shed light on unclear points concerning the mimivirus replication cycle. We found that treatment with cytochalasin, a phagocytosis inhibitor, negatively impacted the incorporation of mimivirus particles by Acanthamoeba castellanii, causing a negative effect on viral growth in amoeba monolayers. Treatment of amoebas with bafilomicin significantly impacted mimivirus uncoating and replication. In conjunction with microscopic analyses, these data suggest that mimiviruses indeed depend on phagocytosis for entry into amoebas, and particle uncoating (and stargate opening) appears to be dependent on phagosome acidification. In-depth analyses of particle morphogenesis suggest that the mimivirus capsids are assembled from growing lamellar structures. Despite proposals from previous studies that genome acquisition occurs before the acquisition of fibrils, our results clearly demonstrate that the genome and fibrils can be acquired simultaneously. Our data suggest the existence of a specific area surrounding the core of the viral factory where particles acquire the surface fibrils. Furthermore, we reinforce the concept that defective particles can be formed even in the absence of virophages. Our work provides new information about unexplored steps in the life cycle of mimivirus. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Imunopatologia de Doenças Virais. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | URMITE. Aix Marseille Universite, Marseille, France | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brazil | pt_BR |
| Subject | mimivirus | pt_BR |
| Subject | electron microscopy | pt_BR |
| Subject | replication cycle | pt_BR |
| Subject | phagocytosis | pt_BR |
| Subject | fibril acquisition área | pt_BR |
| Embargo date | 2022-01-01 | |
