Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/27053
Title: Using next-generation sequencing (NGS) to uncover diversity of wood-decaying fungi in neotropical Atlantic forests
Authors: Vaz, Aline Bruna Martins
Fonseca, Paula Luize Camargos
Leite, Laura Rabelo
Badotti, Fernanda
Salim, Anna Christina de Matos
Araujo, Flavio Marcos Gomes
Cuadros-Orellana, Sara
Duarte, Ângelo A.
Rosa, Carlos Augusto
Oliveira, Guilherme Correa de
Góes Neto, Aristóteles
Affilliation: Universidade Federal de Minas Gerais. Departamento de Microbiologia. Belo Horizonte, MG, Brazil/Faculdade de Minas. Belo Horizonte, MG, Brazil.
Universidade Federal de Minas Gerais. Departamento de Microbiologia. Belo Horizonte, MG, Brazil
Fundação Oswaldo Cruz. Instituto Rene Rachou. Belo Horizonte, MG, Brazil.
Centro Federal de Educação Tecnologica de Minas. Departamento de Quimica. Belo Horizonte, MG, Brazil.
Fundação Oswaldo Cruz. Instituto Rene Rachou. Belo Horizonte, MG, Brazil.
Fundação Oswaldo Cruz. Instituto Rene Rachou. Belo Horizonte, MG, Brazil.
Fundação Oswaldo Cruz. Instituto Rene Rachou. Belo Horizonte, MG, Brazil.
Universidade Estadual de Feira de Santana. Departamento de Tecnologia. Feira de Santana, BA, Brazil.
Universidade Federal de Minas Gerais. Departamento de Microbiologia. Belo Horizonte, MG, Brazil
Vale Instituto de Tecnologia. Desenvolvimento Sustentável. Belém, PA, Brazil.
Universidade Federal de Minas Gerais. Departamento de Microbiologia. Belo Horizonte, MG, Brazil/Faculdade de Minas. Belo Horizonte, MG, Brazil.
Abstract: A targeted amplicon-based metagenomics approach (metabarcoding) provides detailed access to the diversity of the mycobiome in any substrate in distinct environments on Earth. Fungi are the main decomposers of lignocellulosic woody debris in terrestrial forested ecosystems, contributing significantly to the global carbon cycle. The main objectives of this study were to assess the fungal taxonomic diversity in fallen woody debris samples from two Neotropical forest fragments (rainforest and seasonal forest), to analyze the qualitative and quantitative components of the taxonomic diversity, and to investigate the functional diversity of the ecological groups detected. Our study comprised three main methodological steps: (i) sampling in the field; (ii) extraction of DNA, amplification of targeted segments and massively parallel sequencing; and (iii) data analysis and interpretation. A total of 110 molecular operational taxonomic units showing sequence similarity of 95% or more across the two collection sites using two DNA metabarcoding markers (ITS1 and ITS2) were assigned to putative fungal genera in 59 families, 27 orders, and 3 phyla. The number of putative fungal genera and the relative abundance of reads for each genus are higher in the tropical rainforest site than in the tropical seasonal forest site. Most of the identified genera are ligninolytic and cellulolytic and/or hemicellulolytic Basidiomycota (Agaricomycetes) and Ascomycota (Sordariomycetes), but “sugar fungi” and fungi associated with plants and detritivorous insects were also detected. This is the first study using NGS as a rapid and large-scale useful strategy to uncover the diversity of wood-decaying fungi in tropical forests.
Keywords: fungal metabarcoding
rainforest
seasonal tropical forest
lignocellulosic residues
keywords: metabarcode fúngico
floresta tropical
resíduos lignocelulósicos
Issue Date: 2017
Publisher: Magnolia Press
Citation: VAZ, Aline Bruna Martins et al. Using next-generation sequencing (NGS) to uncover diversity of wood-decaying fungi in neotropical Atlantic forests. Phytotaxa, v. 295, n. 1, p. 1-21, 2017
DOI: 10.11646/phytotaxa.295.1.1
ISSN: 1179-3155
Copyright: restricted access
Appears in Collections:MG - IRR - Artigos de Periódicos

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