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Title: Usefulness of PCR-based assays to assess drug efficacy in Chagas disease chemotherapy: value and limitations
Authors: Britto, Constança Carvalho
Affilliation: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ. Brasil.
Abstract: One major goal of research on Chagas disease is the development of effective chemotherapy to eliminate the infection from individuals who have not yet developed cardiac and/or digestive disease manifestations. Cure evaluation is the more complex aspect of its treatment, often leading to diverse and controversial results. The absence of reliable methods or a diagnostic gold standard to assess etiologic treatment efficacy still constitutes a major challenge. In an effort to develop more sensitive tools, polymerase chain reaction (PCR)-based assays were introduced to detect low amounts of Trypanosoma cruzi DNA in blood samples from chagasic patients, thus improving the diagnosis and follow-up evaluation after chemotherapy. In this article, I review the main problems concerning drug efficacy and criteria used for cure estimation in treated chagasic patients, and the work conducted by different groups on developing PCR methodologies to monitor treatment outcome of congenital infections as well as recent and late chronic T. cruzi infections.
Keywords: Chagas Disease
Trypanosoma cruzi
molecular diagnosis
cure assessment
keywords: Doença de Chagas
diagnóstico molecular
Trypanosoma cruzi
avaliação de cura
Issue Date: 2009
Publisher: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz
Citation: BRITTO, Constança Carvalho. Mem Inst Oswaldo Cruz, v..104, p.122-135, Suppl. I, 2009.
ISSN: 0074-0276
Copyright: open access
Appears in Collections:IOC - Artigos de Periódicos

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