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Title: The impact of aging and Zika virus infection on Aedes aegypti female fecundity
Advisor: Freitas, Rafael Maciel de
Authors: Petersen, Martha Thieme
Affilliation: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.
Abstract: The emergence and re-emergence of arboviral diseases, have characterized a Strong global health problematic. Zika virus have reaches the Americas in 2014, gaining more visibility after being associated with microcephaly in newborns and other neurological complications. Its transmission on the continent is mainly carried by females of the species Aedes aegypti, a species that lives very closely to the human population in the cities. For other vector-pathogen models, there has already been observed a fitness cost caused by the viral infection, additional to a negative impact caused by ageing over several life-history traits of the mosquito, such as fecundity with a decrease in the number of eggs laid as they grow older. However, the effects of senescence and ZIKV infection on Ae. aegypti biology remains very underexploited so far, and it\2019s still unknown whether the decreased fecundity is due to aging itself and an inability to produce eggs, or due to a lesser consumption of blood as they age. Therefore, this experiment aimed to investigate the impact of aging, blood meal size and ZIKV infection over the mosquito fecundity and oviposition success, additionally observing the effects of infection over the mosquito daily survival. For that, we divided the experiment in 2 groups, one that would receive a first infected blood meal and the other a first uninfected blood meal, each of these groups divided into 3 cohorts regarding the age at which they would receive their first meal (7, 14 and 21 days old) These mosquitoes were kept with 10% sugar solution cottons every day and blood fed every week with uninfected blood, they were monitored daily for survival and weekly for fecundity, oviposition success and blood meal size by the quantification of hematin. Using ANOVA, we observed a strong negative impact of ZIKV infection over the mosquito\2019s survival. Effects over oviposition success were analyzed by logistic regression, showing that ZIKV infection has a negative effect over this parameter and even though the age of first blood meal did not affect the uninfected groups, the success from the ZIKV cohorts dropped from 76.1% on the first cohort to 59.3% in the third cohort. Clutch size and size of blood meal were analyzed by using a repeated measure analysis, having number of eggs and hematin amounts as the repeated measures. While the blood meal size did not influence clutch size, it was strongly influenced by age in the uninfected group, becoming less efficient to produce eggs per \03BCL of blood ingested, clutch size sharply decreasing with age. On the other hand, the infected group showed a larger ingestion of blood than the uninfected and a more stable production of eggs per \03BCL of blood ingested, fecundity even increasing over time. Therefore, our results try to elucidate aspects of vectorial capacity by observing the effects of senescence and pathogen infection over Aedes aegypti life history traits.
keywords: Aedes
Zika Vírus
Issue Date: 2018
Citation: PETERSEN, Martha Thieme. The impact of aging and Zika virus infection on Aedes aegypti female fecundity. 2018. 72 f. Dissertação (Mestrado em Biologia Parasitária)-Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, 2018.
Date of defense: 2018
Place of defense: Rio de Janeiro
Department: Instituto Oswaldo Cruz
Defense institution: Fundação Oswaldo Cruz
Program: Programa de Pós-Graduação em Biologia Parasitária
Copyright: open access
Appears in Collections:IOC - PGBP - Dissertações de Mestrado

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