Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/27495
Title: Evaluation of the antiplasmodial and leishmanicidal potential of Myrciaria dubia (Myrtaceae) extract.
Authors: Correia, Vanessa Carolina de Sena.
Lima, Nathália Oliveira.
Oliveira, Flávio Augusto de Souza.
Santos, Ana Paula de Azevedo dos.
Teles, Carolina Bioni Garcia.
Júnior, Waldesse Piragé de Oliveira.
Pimenta, Raphael Sanzio.
Affilliation: Universidade Federal do Tocantins. Laboratório de Microbiologia Geral e Aplicada. Palmas, TO, Brasil. / Universidade Federal do Tocantins. Laboratório de Biotecnologia. Palmas, TO, Brasil.
Universidade Federal do Tocantins. Laboratório de Biotecnologia. Palmas, TO, Brasil.
Fundação Oswaldo Cruz. Laboratório da Plataforma de Bioensaios de Malária e Leishmaniose. Porto Velho, RO, Brasil.
Fundação Oswaldo Cruz. Laboratório da Plataforma de Bioensaios de Malária e Leishmaniose. Porto Velho, RO, Brasil.
Fundação Oswaldo Cruz. Laboratório da Plataforma de Bioensaios de Malária e Leishmaniose. Porto Velho, RO, Brasil.
Universidade Federal do Tocantins. Laboratório de Biotecnologia. Palmas, TO, Brasil.
Universidade Federal do Tocantins. Laboratório de Microbiologia Geral e Aplicada. Palmas, TO, Brasil.
Abstract: Introduction: Malaria and leishmaniasis are prevalent in tropical regions, which have environmental characteristics that are highly favorable to protozoa and vectors of these diseases; the transmission of these infections in sub-tropical regions, although recognized, represents only a small fraction of cases. Plants are constantly being used in the search for and acquisition of new drugs, and many compounds derived from them have been used to combat various diseases. In this study, we evaluated the action of the dichloromethanolic extract of Myrciaria dubia leaves against the protozoa Plasmodium falciparum, Leishmania amazonensis, Leishmania braziliensis, and Leishmania chagasi through bioassays. Methods: The extract from M. dubia was tested for its anti-P. falciparum activity in an anti-histidine-rich protein II immunosorbent assay. The antileishmanial assays were performed using the resazurin method, while cytotoxicity against human hepatoma (HepG2) strain was determined using the colorimetric MTT [3-(4, 5-dimethyl-2- thiazolyl)-2, 5-diphenyl-2H tetrazolium bromide] method. Results: The M. dubia extract presented a half-maximal inhibitory concentration equal to 2.35 (1.05)μg/mL for P. falciparum, 190.73 (6.41) μg/mL for L. amazonensis, and greater than equal to 200μg/mL for L. chagasi and L. braziliensis strains. The cytotoxic concentration for 50% of the cells was above 500μg/mL for HepG2, indicating no toxicity and greater selectivity against parasites. Conclusions: The results obtained indicate the presence of antiplasmodial and leishmanicidal bioactive compounds in the dichloromethanolic extracts of M. dubia leaves, and point towards future studies to elucidate the mechanism of action for each physiological effect.
Keywords: Plasmodium falciparum.
Leishmania spp.
Camu-camu.
Canguçu.
Issue Date: 2016
Publisher: Revista da Sociedade Brasileira de Medicina Tropical
Citation: TELES, C.B.G. et al. Evaluation of the antiplasmodial and leishmanicidal potential of Myrciaria dubia (Myrtaceae) extract. Sociedade Brasileira de Medicina Tropical, v. 49, p. 586-592, 2016.
ISSN: 0037-8682
Copyright: open access
Appears in Collections:RO - Artigos de Periódicos



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