Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/27499
Title: Cellular immune response profile in patients with American tegumentary leishmaniasis prior and post chemotherapy treatment
Authors: Reis, Luiza C.
Brito, Maria Edilenza F.
Souza, Marina A.
Medeiros, Angela C. R.
Silva, Claudio J.
Luna, Carlos F.
Pereira, Valéria R. A.
Affilliation: Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil.
Universidade de Pernambuco/UPE. Hospital Oswaldo Cruz—HUOC. Recife, PE, Brazil.
Núcleo de Vigilância em Saúde e Ambiente do Município de Moreno. Moreno, PE, Brazil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil.
Abstract: In this study, we have the objective of evaluating the lymphoproliferative response and determining interferon (IFN)-gamma and interleukin (IL)-10 cytokine production in the peripheral blood mononuclear cells (PBMC) of patients with American tegumentary leishmaniasis prior and post 12 months of chemotherapy treatment with meglumine antimoniate compared with the PBMC of noninfected donors. Lymphoproliferation, such as cytokine production, was evaluated through in vitro stimulus with the soluble antigenic fraction from Leishmania (Viannia) braziliensis promastigotes (1.25 microg/ml) and Concanavalin A (2.5 microg/ml). Patients showed a significant lymphoproliferative response prior and post treatment compared with the control group. Similar result, prior to chemotherapy treatment, was observed in IFN-gamma and IL-10 production when patients were compared with the control group. After chemotherapy treatment, PBMC lymphoproliferative response of the patients revealed an increase, whereas patients have shown a decrease in IFN-gamma levels and an increase in IL-10, although without statistical difference. These results may indicate that the patients produced a specific cellular response to the soluble antigenic fraction suggesting that besides Th1 and Th2 dichotomy, immunological regulation mechanisms with the participation of memory T cells and regulatory T cells could be present in the clinical evolution of these patients. This understanding will allow the study and identification of new L. (V.) braziliensis molecules potentially candidates to vaccines.
Keywords: American tegumentary leishmaniasis
Leishmania (Viannia) braziliensis
Cytokines
Chemotherapy treatment
T lymphocytes
keywords: Leishmaniose tegumentar americana
Leishmania (Viannia) braziliensis
Citocinas
Tratamento quimioterápico
Linfócitos T
DeCS: Animais
Antígenos, Protozoários / imunologia
Estudos de caso-controle
Concanavalina A / farmacologia
Fêmea
Estudos de Acompanhamento
Humanos
Imunidade Celular / fisiologia
Interferon gama / análise
Interferon gama / imunologia
Interleucina-10 / análise
Interleucina-10 / imunologia
Leishmania braziliensis / imunologia
Leishmaniose cutânea / quimioterapia
Leishmaniose cutânea / imunologia
Leishmaniose cutânea / parasitologia
Leucócitos Mononucleares / efeitos de drogas
Leucócitos Mononucleares / imunologia
Masculino
Meglumina / uso terapêutico
Compostos Organometálicos / uso terapêutico
Estatísticas não paramétricas
Resultado do tratamento
Issue Date: 2009
Citation: REIS, L. C. et al. Cellular immune response profile in patients with American tegumentary leishmaniasis prior and post chemotherapy treatment. Journal of Clinical Laboratory Analysis, v. 23, n. 1, p. 63–69, 2009.
DOI: 10.1002/jcla.20291
ISSN: 1098-2825
Copyright: restricted access
Appears in Collections:PE - IAM - Artigos de Periódicos
PE - IAM - Artigos de Periódicos

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