Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/2768
Title: Genetic diversity of Leishmania amazonensis strains isolated in northeastern Brazil as revealed by DNA sequencing, PCR-based analyses and molecular karyotyping
Authors: Oliveira, João Paulo C. de
Fernandes, Flora Maria de Campos
Cruz, Angela Kaysel
Trombela, Viviane
Monteiro, Elisângela
Camargo, Anamaria A
Barral, Aldina Maria Prado
Oliveira, Camila Indiani de
Affilliation: Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Ribeirão Preto, SP, Brasil
Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Ribeirão Preto, SP, Brasil
Ludwig Institute for Cancer Research. São Paulo, SP, Brasil
Ludwig Institute for Cancer Research. São Paulo, SP, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Abstract: Background: Leishmania (Leishmania) amazonensis infection in man results in a clinical spectrum of disease manifestations ranging from cutaneous to mucosal or visceral involvement. In the present study, we have investigated the genetic variability of 18 L. amazonensis strains isolated in northeastern Brazil from patients with different clinical manifestations of leishmaniasis. Parasite DNA was analyzed by sequencing of the ITS flanking the 5.8 S subunit of the ribosomal RNA genes, by RAPD and SSR-PCR and by PFGE followed by hybridization with gene-specific probes. Results: ITS sequencing and PCR-based methods revealed genetic heterogeneity among the L. amazonensis isolates examined and molecular karyotyping also showed variation in the chromosome size of different isolates. Unrooted genetic trees separated strains into different groups. Conclusion: These results indicate that L. amazonensis strains isolated from leishmaniasis patients from northeastern Brazil are genetically diverse, however, no correlation between genetic polymorphism and phenotype were found.
Keywords: Genetic Diversity
Strains
Leishmania amazonensis
Brazil
Northeastern
DNA
Sequencing
Molecular karyotyping
PCR
keywords: Diversidade Genética
Cepas
Leishmania amazonensis
Brasil
Nordeste
DNA
Sequenciamento
Cariotipagem molecular
PCR
DeCS: Variação Genética
Leishmania
Brasil
Análise de Sequência de DNA
Cariotipagem
Reação em Cadeia da Polimerase
Issue Date: 2007
Citation: OLIVEIRA, J. P. C. de et al. Genetic diversity of Leishmania amazonensis strains isolated in northeastern Brazil as revealed by DNA sequencing, PCR-based analyses and molecular karyotyping. Kinetoplastid Biology and Disease, v.6, n.5, p.1-8, 2007.
Description: Agradecemos a Lucile Floeter-Winter pela leitura crítica do manuscrito e Artur T.L. de Queiroz para ajuda inicial com a análise filogenética.
DOI: 10.1186/1475-9292-6-5
ISSN: 1475-9292
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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