Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/27781
Title: The redox-sensing gene Nrf2 affects intestinal homeostasis, insecticide resistance, and Zika virus susceptibility in the mosquito Aedes aegypti
Authors: Bottino-Rojas, Vanessa
Talyuli, Octavio A. C.
Carrara, Luana
Martins, Ademir J.
James, Anthony A.
Oliveira, Pedro L.
Silva, Gabriela O. Paiva
Affilliation: Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Fisiologia e Controle de Artrópodes Vetores. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Fisiologia e Controle de Artrópodes Vetores. Rio de Janeiro, RJ. Brasil / Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular. Rio de Janeiro, RJ, Brasil.
University of California. Departments of Microbiology & Molecular Genetics and Molecular Biology & Biochemistry. Irvine, CA, USA.
Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular. Rio de Janeiro, RJ, Brasil.
Abstract: Production and degradation of reactive oxygen species (ROS) are extensively regulated to ensure proper cellular responses to various environmental stimuli and stresses. Moreover, physiologically generated ROS function as secondary messengers that can influence tissue homeostasis. The cap'n'collar transcription factor known as nuclear factor erythroid-derived factor 2 (Nrf2) coordinates an evolutionarily conserved transcriptional activation pathway that mediates antioxidant and detoxification responses in many animal species, including insects and mammals. Here, we show that Nrf2-mediated signaling affects embryo survival, midgut homeostasis, and redox biology in Aedes aegypti, a mosquito species vector of dengue, Zika, and other disease-causing viruses. We observed that AeNrf2 silencing increases ROS levels and stimulates intestinal stem cell proliferation. Because ROS production is a major aspect of innate immunity in mosquito gut, we found that a decrease in Nrf2 signaling results in reduced microbiota growth and Zika virus infection. Moreover, we provide evidence that AeNrf2 signaling also controls transcriptional adaptation of A. aegypti to insecticide challenge. Therefore, we conclude that Nrf2-mediated response regulates assorted gene clusters in A. aegypti that determine cellular and midgut redox balance, affecting overall xenobiotic resistance and vectorial adaptation of the mosquito.
Keywords: nuclear factor 2
erythroid‐derived 2‐like factor
Nrf2
cap`n`collor
NFE2L2
oxidative stress
stem cell
Zika Virus
insecticide resistance
keywords: resistência a inseticidas
Zika Virus
Aedes aegypti
fator nuclear 2
célula tronco
estresse oxidativo
Fator 2 Relacionado a NF-E2
Issue Date: 2018
Publisher: SAGE Publications
Citation: BOTTINO-ROJAS, Vanessa; et al. The redox-sensing gene Nrf2 affects intestinal homeostasis, insecticide resistance and Zika virus susceptibility in the mosquito Aedes aegypti. JBC Papers in Press, Apr.2018.
DOI: 10.1074/jbc.RA117.001589
ISSN: 0021-9436
Copyright: open access
Appears in Collections:IOC - Artigos de Periódicos

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