Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/27812
Title: Hospitalization due to norovirus and genotypes of rotavirus in pediatric patients, state of Espírito Santo
Authors: Ribeiro, Leandro Rua
Giuberti, Rafael Souto de Oliveira
Barreira, Débora Maria Pires Gonçalves
Saick, Ketene Wernek
Leite, José Paulo Gagliardi
Miagostovich, Marize Pereira
Spano, Liliana Cruz
Affilliation: Universidade Federal do Espírito Santo. Centro Biomédico. Departamento de Patologia. Núcleo de Doenças Infecciosas. Vitória, ES, Brasil.
Universidade Federal do Espírito Santo. Centro Biomédico. Departamento de Patologia. Núcleo de Doenças Infecciosas. Vitória, ES, Brasil.
Universidade Federal do Espírito Santo. Centro Biomédico. Departamento de Patologia. Núcleo de Doenças Infecciosas. Vitória, ES, Brasil.
Universidade Federal do Espírito Santo. Centro Biomédico. Departamento de Patologia. Núcleo de Doenças Infecciosas. Vitória, ES, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada. Rio de Janeiro, RJ. Brasil.
Universidade Federal do Espírito Santo. Centro Biomédico. Departamento de Patologia. Núcleo de Doenças Infecciosas. Vitória, ES, Brasil.
Abstract: Viruses are the leading cause for hospitalization due to gastroenteritis worldwide. Group A rotaviruses (RV) are the most prevalent and are assorted in glycoproteins (G) and protease sensitive (P) dual genotypes based on polymorphic genes that encode the external VP7 and VP4 capsid proteins, respectively. Noroviruses (NoV) have increasingly answered by sporadic gastroenteritis. This study aimed to determine the prevalence of NoV and RV in 68 hospitalized children, between July 2004 and November 2006, at a pediatric hospital in Vitória city, state of Espírito Santo, Southeastern Brazil. Nucleic acid was extracted from fecal suspension following the guanidine-silica procedure. Reverse transcriptase-polymerase chain reaction (RT-PCR) and polyacrylamide gel electrophoresis were employed for NoV and RV detection, respectively. RV genotyping was accomplished using RT-PCR followed by heminested multiplex PCR with specific primers for the most prevalent types of G and P. Fecal samples were positive for NoV and RV in 39.7% (27/68) and 20.5% (14/68), respectively and together were responsible for 60% (41/68) of the cases. RV genotypes were: 50% G9P[8], 28.7% G2P[4], 7.1% G1P[8], G2P[8] and G?P[8]. Vomit was a prominent manifestation observed in 92% and 85% of the NoV and RV cases, respectively. The median hospitalization was 5 and 5.5 days for the patients infected with NoV and RV, respectively. The data showed that NoV prevailed over RV and it also corroborated the emergence of RV G9 genotype followed by G2P[4], reinforcing the need for RV genotype surveillance.
Keywords: noroviruses
group A rotaviruses
sporadic gastroenteritis
hospitalizations
keywords: norovírus
gastroenterite esporádica
rotavírus do grupo A
hospitalizações
Issue Date: 2008
Publisher: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz
Citation: RIBEIRO, Leandro Rua; et al. Hospitalization due to norovirus and genotypes of rotavirus in pediatric patients, state of Espírito Santo. Mem Inst Oswaldo Cruz, v.103, n.2, p. 201-206, Mar. 2008.
ISSN: 0074-0276
Copyright: open access
Appears in Collections:IOC - Artigos de Periódicos

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