Author | Ferreira, F. F. M. | |
Author | Guimarães, F. R. | |
Author | Fumian, T. M. | |
Author | Victoria, M. | |
Author | Vieira, C. B. | |
Author | Luz, S. | |
Author | Shubo, T. | |
Author | Leite, José Paulo Gagliardi | |
Author | Miagostovich, Marize Pereira | |
Access date | 2018-08-07T13:53:54Z | |
Available date | 2018-08-07T13:53:54Z | |
Document date | 2009 | |
Citation | FERREIRA, F. F. M. et al. Environmental dissemination of group A rotavirus: P-type, G-type and subgroup characterization. Water Science & Technology, v.60, n.3, p.633-642, 2009. | pt_BR |
ISSN | 0273-1223 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/27977 | |
Language | eng | pt_BR |
Publisher | IWA Publishing | pt_BR |
Rights | restricted access | pt_BR |
Subject in Portuguese | Águas residuais | pt_BR |
Subject in Portuguese | Rotavírus do grupo A | pt_BR |
Subject in Portuguese | água do rio | pt_BR |
Title | Environmental dissemination of group A rotavirus: P-type, G-type and subgroup characterization | pt_BR |
Type | Article | pt_BR |
DOI | 10.2166/wst.2009.413 | |
Abstract | Rotaviruses A (RV-A) infection is the most common cause of acute diarrheal diseases in infants and the dissemination of these viruses in the environment represents a public health hazard. The present study aims to evaluate reverse transcription-polymerase chain reaction (RT-PCR) based protocols for the detection of RV-A genes in different types of environmental samples. RV-A were concentrated by the adsorption-elution method using negatively charged membranes associated with a Centriprep Concentrator 50. The RV-A VP4, VP7 and VP6 genes were detected using RT-PCR in river water from the Amazon Hydrographic basin (Northern region) and from wastewater in a sewage treatment plant in Rio de Janeiro (Southeast region), Brazil. RV-A were successfully detected in water environmental samples by the methods used. The detection of the VP6 gene by RT-PCR was the most sensitive for detecting RV-A in environmental samples (44.0%), when compared to the detection of the VP4 (33.3%) and VP7 (25.3%) genes. Based on nucleotide sequence and phylogenetic analysis of the partial VP6 gene, 22 environmental samples were determined to be subgroup II (Wa-like). These results indicate that analysis of environmental samples could possibly make a valuable contribution to studies on the epidemiology of RV-A. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ. Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ. Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ. Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ. Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ. Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Leônidas e Maria Deane. Biodiversidade em Saúde. Manaus, AM, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Diretoria de Administração do Campus Rio de Janeiro. Assessoria Técnica de Infra-estrutura e Meio Ambiente Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ. Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ. Brasil. | pt_BR |
Subject | river water | pt_BR |
Subject | Rotavirus A | pt_BR |
Subject | VP4 | pt_BR |
Subject | VP6 | pt_BR |
Subject | VP7 | pt_BR |
Subject | wastewater | pt_BR |
e-ISSN | 1996-9732 | |
Embargo date | 2030-01-01 | |