Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/2802
Full metadata record
DC FieldValue
dc.contributor.authorMenezes, Maria José
dc.contributor.authorCosta, Dirceu Joaquim
dc.contributor.authorClarêncio, Jorge
dc.contributor.authorMiranda, José Carlos
dc.contributor.authorBarral, Aldina Maria Prado
dc.contributor.authorBarral Netto, Manoel
dc.contributor.authorBrodskyn, Claudia Ida
dc.contributor.authorOliveira, Camila Indiani de
dc.date.accessioned2011-08-20T22:35:31Z
dc.date.available2011-08-20T22:35:31Z
dc.date.issued2008
dc.identifier.citationMENEZES, M. J. et al. Immunomodulation of human monocytes following exposure to Lutzomyia intermedia saliva. BMC Immunology, v. 9, n. 12, p. 1-8, 2008
dc.identifier.issn1471-2172
dc.identifier.urihttps://www.arca.fiocruz.br/handle/icict/2802
dc.description.sponsorshipThis work was supported by grants from CNPq, FAPESB and PAPES/FIOCRUZ. M.J.M. was supported by a CAPES fellowship;
dc.language.isoeng
dc.rightsopen access
dc.titleImmunomodulation of human monocytes following exposure to Lutzomyia intermedia saliva
dc.typeArticle
dc.identifier.doi10.1186/1471-2172-9-12
dc.description.abstractenSand fly saliva contains potent and complex pharmacologic molecules that are able to modulate the host's hemostatic, inflammatory, and immune systems. In this study, we evaluated the effects of salivary gland sonicate (SGS) of Lutzomyia intermedia, the natural vector of Leishmania braziliensis, on monocytes obtained from the peripheral blood mononuclear cells (PBMC) of healthy volunteers. We investigated the effects of sand fly saliva on cytokine production and surface molecule expression of LPS-stimulated human monocytes uninfected or infected with L. braziliensis.Pre-treatment of non-infected human monocytes with L. intermedia SGS followed by LPS-stimulation led to a significant decrease in IL-10 production accompanied by a significant increase in CD86, CD80, and HLA-DR expression. Pre-treatment with SGS followed by LPS stimulation and L. braziliensis infection led to a significant increase in TNF-alpha, IL-6, and IL-8 production without significant alterations in co-stimulatory molecule expression. However, pre-treatment with L. intermedia SGS did not result in significant changes in the infection rate of human monocytes.Our data indicate that L. intermedia saliva is able to modulate monocyte response, and, although this modulation is dissociated from enhanced infection with L. braziliensis, it may be associated with successful parasitism
dc.creator.affilliationFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz, Salvador, BA. Brasil
dc.creator.affilliationFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz, Salvador, BA. Brasil
dc.creator.affilliationFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz, Salvador, BA. Brasil
dc.creator.affilliationFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz, Salvador, BA. Brasil
dc.creator.affilliationFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz, Salvador, BA. Brasil
dc.creator.affilliationFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz, Salvador, BA. Brasil
dc.creator.affilliationFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz, Salvador, BA. Brasil
dc.creator.affilliationFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz, Salvador, BA. Brasil
dc.subject.decsLeishmaniose
dc.subject.decsLeishmania braziliensis
dc.subject.decsMonócitos
dc.subject.decsPsychodidae
dc.subject.decsSaliva
dc.subject.decsCitocinas
dc.subject.decsCitometria deFluxo
Appears in Collections:BA - IGM - Artigos de Periódicos

Files in This Item:
File Description SizeFormat 
P Immunomodulation of human monocytes following exposure.pdf447.5 kBAdobe PDFThumbnail
View/Open


FacebookTwitterDeliciousLinkedInGoogle BookmarksBibTex Format mendeley Endnote DiggMySpace

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.