Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/28459
Title: Activated dendritic cells modulate proliferation and differentiation of human myoblasts
Authors: Ladislau, Leandro
Portilho, Débora M.
Courau, Tristan
Solares-Pérez, Alhondra
Negroni, Elisa
Lainé, Jeanne
Klatzmann, David
Bonomo, Adriana
Allenbach, Yves
Benveniste, Olivier
Riederer, Ingo
Savino, Wilson
Mouly, Vincent
Butler-Browne, Gillian
Benjamim, Claudia F.
Affilliation: Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil / Sorbonne Université. Institut fr Myologie. Paris, France.
Sorbonne Université. Institut de Myologie. Paris, France.
Sorbonne Université. Immunology-Immunopathology-Immunotherapy. Paris, France.
Sorbonne Université. Institut de Myologie. Paris, France.
Sorbonne Université. Institut de Myologie. Paris, France.
Sorbonne Université. Institut de Myologie. Paris, France.
Sorbonne Université. Immunology-Immunopathology-Immunotherapy. Paris, France.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ. Brasil.
Sorbonne Université. Institut de Myologie. Paris, France.
Sorbonne Université. Institut de Myologie. Paris, France.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ. Brasil / Instituto Nacional de Ciência e Tecnologia em Neuroimunomodulação. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ. Brasil / Instituto Nacional de Ciência e Tecnologia em Neuroimunomodulação. Rio de Janeiro, RJ, Brasil.
Sorbonne Université. Institut de Myologie. Paris, France.
Sorbonne Université. Institut de Myologie. Paris, France.
Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.
Abstract: Idiopathic Inflammatory Myopathies (IIMs) are a heterogeneous group of autoimmune diseases affecting skeletal muscle tissue homeostasis. They are characterized by muscle weakness and inflammatory infiltration with tissue damage. Amongst the cells in the muscle inflammatory infiltration, dendritic cells (DCs) are potent antigen-presenting and key components in autoimmunity exhibiting an increased activation in inflamed tissues. Since, the IIMs are characterized by the focal necrosis/regeneration and muscle atrophy, we hypothesized that DCs may play a role in these processes. Due to the absence of a reliable in vivo model for IIMs, we first performed co-culture experiments with immature DCs (iDC) or LPS-activated DCs (actDC) and proliferating myoblasts or differentiating myotubes. We demonstrated that both iDC or actDCs tightly interact with myoblasts and myotubes, increased myoblast proliferation and migration, but inhibited myotube differentiation. We also observed that actDCs increased HLA-ABC, HLA-DR, VLA-5, and VLA-6 expression and induced cytokine secretion on myoblasts. In an in vivo regeneration model, the co-injection of human myoblasts and DCs enhanced human myoblast migration, whereas the absolute number of human myofibres was unchanged. In conclusion, we suggest that in the early stages of myositis, DCs may play a crucial role in inducing muscle-damage through cell-cell contact and inflammatory cytokine secretion, leading to muscle regeneration impairment.
Keywords: Activated dendritic cells
human mioblasts
proliferation
differentiation
Idiopathic Inflammatory Myopathies
keywords: Células dendríticas ativadas
mioblastos humanos
proliferação
diferenciação
Miopatias Inflamatórias Idiopáticas
Issue Date: 2018
Publisher: Nature Publishing Group
Citation: LADISLAU, Leandro; et al. Activated dendritic cells modulate proliferation and differentiation of human myoblasts. Cell Death and Disease, v.9:551, 14p, 2018.
DOI: 10.1038/s41419-018-0426-z
ISSN: 2041-4889
Copyright: open access
Appears in Collections:IOC - Artigos de Periódicos

Files in This Item:
File Description SizeFormat 
ingo_riederer_etal_IOC_2018.pdf4.76 MBAdobe PDFView/Open


FacebookTwitterDeliciousLinkedInGoogle BookmarksBibTex Format mendeley Endnote DiggMySpace

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.