Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/28696
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dc.contributor.authorCavalcanti, Danielle Pereira
dc.contributor.authorShimada, Márcia Kiyoe
dc.contributor.authorProbst, Christian Macagnan
dc.contributor.authorSouto-Padrón, Thais Cristina Baeta Soares
dc.contributor.authorSouza, Wanderley de
dc.contributor.authorGoldenberg, Samuel
dc.contributor.authorFragoso, Stenio Perdigão
dc.contributor.authorMotta, Maria Cristina Machado
dc.date.accessioned2018-09-12T18:01:15Z
dc.date.available2018-09-12T18:01:15Z
dc.date.issued2009
dc.identifier.citationCAVALCANTI, Danielle Pereira et al. Expression and subcellular localization of kinetoplast-associated proteins in the different developmental stages of Trypanosoma cruzi. BMC Microbiology, v. 9, n. 120, 2009.
dc.identifier.issn1471-2180
dc.identifier.urihttps://www.arca.fiocruz.br/handle/icict/28696
dc.language.isopor
dc.publisherSpringer Nature
dc.rightsopen access
dc.subject.otherTrypanosoma cruzi
dc.subject.otherCrithidia fasciculata
dc.titleExpression and subcellular localization of kinetoplast-associated proteins in the different developmental stages of Trypanosoma cruzi
dc.typeArticle
dc.identifier.doi10.1186/1471-2180-9-120
dc.description.abstractenhe kinetoplast DNA (kDNA) of trypanosomatids consists of an unusual arrangement of circular molecules catenated into a single network. The diameter of the isolated kDNA network is similar to that of the entire cell. However, within the kinetoplast matrix, the kDNA is highly condensed. Studies in Crithidia fasciculata showed that kinetoplast-associated proteins (KAPs) are capable of condensing the kDNA network. However, little is known about the KAPs of Trypanosoma cruzi, a parasitic protozoon that shows distinct patterns of kDNA condensation during their complex morphogenetic development. In epimastigotes and amastigotes (replicating forms) the kDNA fibers are tightly packed into a disk-shaped kinetoplast, whereas trypomastigotes (non-replicating) present a more relaxed kDNA organization contained within a rounded structure. It is still unclear how the compact kinetoplast disk of epimastigotes is converted into a globular structure in the infective trypomastigotes. In this work, we have analyzed KAP coding genes in trypanosomatid genomes and cloned and expressed two kinetoplast-associated proteins in T. cruzi: TcKAP4 and TcKAP6. Such small basic proteins are expressed in all developmental stages of the parasite, although present a differential distribution within the kinetoplasts of epimastigote, amastigote and trypomastigote forms. This way concluded thas several features of TcKAPs, such as their small size, basic nature and similarity with KAPs of C. fasciculata, are consistent with a role in DNA charge neutralization and condensation. Additionally, the differential distribution of KAPs in the kinetoplasts of distinct developmental stages of the parasite, indicate that the kDNA rearrangement that takes place during the T. cruzi differentiation process is accompanied by TcKAPs redistribution.
dc.creator.affilliationUniversidade Federal do Mato Grosso do Sul. Campo Grande, MS, Brasil.
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.
dc.creator.affilliationUniversidade Católica Dom Bosco. Campo Grande, MS, Brasil.
dc.creator.affilliationUniversidade Federal do Paraná. Curitiba, PR, Brasil.
dc.creator.affilliationUniversidade Federal do Mato Grosso do Sul. Campo Grande, MS, Brasil.
dc.creator.affilliationEmbrapa Gado de Corte. Saúde Animal. Laboratório de Engenharia Genética Animal, Campo Grande, MS, Brasil.
dc.creator.affilliationEmbrapa Gado de Corte. Saúde Animal. Laboratório de Engenharia Genética Animal, Campo Grande, MS, Brasil.
dc.subject.enDNA
dc.subject.enKinetoplast
dc.subject.enCloning
dc.subject.enGene Expression
dc.subject.esADN de Cinetoplasto
dc.subject.esClonación Molecular
dc.subject.esExpresión Génica
dc.subject.decsDNA de Cinetoplasto
dc.subject.decsClonagem Molecular
dc.subject.decsExpressão Gênica
dc.identifier.eissn1471-2180
Appears in Collections:PR - ICC - Artigos de Periódicos

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