Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/29507
Title: Unveiling the oxidative metabolism of Achatina fulica (Mollusca: Gastropoda) experimentally infected to Angiostrongylus cantonensis (Nematoda: Metastrongylidae)
Authors: Tunholi-Alves, Vinícius Menezes
Tunholi, Victor Menezes
Garcia, Juberlan
Mota, Esther Maria
Castro, Rosana Nora
Pontes, Emerson Guedes
Pinheiro, Jairo
Affilliation: Universidade Federal Rural do Rio de Janeiro. Instituto de Biologia. Departamento de Ciências Fisiológicas. Seropédica, RJ, Brasil / Universidade Federal Rural do Rio de Janeiro. Departamento de Parasitologia Animal. Seropédica, RJ, Brasil.
Universidade Federal Rural do Rio de Janeiro. Instituto de Biologia. Departamento de Ciências Fisiológicas. Seropédica, RJ, Brasil / Universidade Federal Rural do Rio de Janeiro. Departamento de Parasitologia Animal. Seropédica, RJ, Brasil
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia e Parasitologia de Mamíferos Silvestres Reservatórios. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Patologia. Rio de Janeiro, RJ. Brasil.
Universidade Federal Rural do Rio de Janeiro. Instituto de Ciências Exatas. Departamento de Química. Seropédica, RJ, Brasil.
Universidade Federal Rural do Rio de Janeiro. Instituto de Ciências Exatas. Departamento de Química. Seropédica, RJ, Brasil.
Universidade Federal Rural do Rio de Janeiro. Instituto de Biologia. Departamento de Ciências Fisiológicas. Seropédica, RJ, Brasil.
Abstract: For the first time, alterations in the oxidative metabolism of Achatina fulica experimentally infected with different parasite loads of Angiostrongylus cantonensis were determined. For this, the hemolymph activities of lactate dehydrogenase (LDH) and hexokinase and the glucose concentrations in the hemolymph, as well as the polysaccharide reserves in the digestive gland and cephalopedal mass, were assessed. Additionally, the contents of some carboxylic acids in the hemolymph of infected and uninfected snails were determined by high-performance liquid chromatography (HPLC), permitting a better understanding of the alterations related to the host’s oxidative metabolism. As the main results, activation of oxidative pathways, such as the glycolytic pathway, was demonstrated in response to the increase in the activity of hexokinase. This tendency was confirmed by the decrease in the contents of glucose in the hemolymph of parasitized snails, indicating that the infection by A. cantonensis alters the host’s metabolism, and that these changes are strongly influenced by the parasite load. This metabolic scenario was accompanied by activation of the anaerobic fermentative metabolism, indicated not only by an increase in the activity of (LDH), but also by a reduction of the content of pyruvic acid and accumulation of lactic acid in the hemolymph of parasitized snails. In this circumstance, maintenance of the host’s redox balance occurs through activation of the fermentative pathways, and LDH plays a central role in this process. Together, the results indicate that A. cantonensis infection induces activation of the anaerobic metabolism of A. fulica, characterized not only by the accumulation of lactic acid, but also by a reduction in the pyruvic acid and oxalic acid contents in the hemolymph of the infected snails.
Keywords: Carboxylic acids
Host-parasite relation
Achatina fulica
Angiostrongylus cantonensis
keywords: Achatina fulica
Angiostrongylus cantonensis
Relação parasita-hospedeiro
Ácidos carboxílicos
Issue Date: 2018
Publisher: Springer Verlag (Germany)
Citation: TUNHOLI-ALVES, Vinícius Menezes; et al. Unveiling the oxidative metabolism of Achatina fulica (Mollusca: Gastropoda) experimentally infected to Angiostrongylus cantonensis (Nematoda: Metastrongylidae). Parasitology Research, v.117, p:1773–1781, Apr. 2018.
ISSN: 0932-0113
Copyright: restricted access
Appears in Collections:IOC - Artigos de Periódicos

Files in This Item:
File Description SizeFormat 
estermaria_motta_etal_IOC_2018.pdf1.33 MBAdobe PDF    Request a copy


FacebookTwitterDeliciousLinkedInGoogle BookmarksBibTex Format mendeley Endnote DiggMySpace

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.