Author | Ferreira, Tamara Aparecida Reis | |
Author | Andrade, Hélida Monteiro de | |
Author | Pádua, Paulo Madureira de | |
Author | Carvalho, Maria das Graças | |
Author | Pires, Simone da Fonseca | |
Author | Oliveira, Ivana Helena Rocha | |
Author | Lima, Bruna Soares Souza | |
Author | Fialho Junior, Luis Carlos | |
Author | Cicarini, Walter Batista | |
Author | Chapeourouge, Donat Alexander | |
Author | Perales, Jonas Henrique | |
Author | Guimarães, Tânia Mara Pinto Dabés | |
Author | Toledo, Vicente de Paulo Coelho Peixoto de | |
Access date | 2018-10-18T12:01:25Z | |
Available date | 2018-10-18T12:01:25Z | |
Document date | 2017 | |
Citation | FERREIRA, Tamara Aparecida Reis; et al. Identification of potential biomarkers for systemic lupus erythematosus diagnosis using two-dimensional differential gel electrophoresis (2D-DIGE) and mass spectrometry. Autoiimunity, v.50, n.4, p.247-256, 2017. | pt_BR |
ISSN | 0891-6934 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/29621 | |
Language | eng | pt_BR |
Publisher | Taylor & Francis | pt_BR |
Rights | restricted access | pt_BR |
Subject in Portuguese | Lúpus eritematoso sistêmico | pt_BR |
Subject in Portuguese | Proteômica | pt_BR |
Subject in Portuguese | isoforma de proteína 4 de ligação ao retinol X1 | pt_BR |
Title | Identification of potential biomarkers for systemic lupus erythematosus diagnosis using twodimensional differential gel electrophoresis (2DDIGE) and mass spectrometry | pt_BR |
Type | Article | pt_BR |
DOI | 10.1080/08916934.2017.1344975 | |
Abstract | Systemic lupus erythematosus (SLE) is an autoimmune disease of the connective tissue with a large
spectrum of clinical manifestations. Immune deregulation leads to autoantibody and immune complexes
overproduction, complement activation, and persistent tissue inflammation. Considering that
the current diagnosis depends on the interpretation of the complex criteria established by the
American College of Rheumatology and that the disease course is characterized by unpredictable activations
and remissions, each patient develops different manifestations, and therefore, the discovery of
specific biomarkers is urgently required. Therefore, this study aimed to identify putative biomarkers for
active and inactive SLE potentially capable in distinguishing laboratorial SLE from other autoimmune
diseases. The 2D-DIGE proteomics technique was used to evaluate the differential abundance of proteins
between patients with active SLE, inactive SLE, patients with other autoimmune disease, and
healthy individuals. Six proteins showed increased abundance in active SLE (A) and inactive SLE (I)
compared to the C and O groups, but not between groups A and I. There were two transthyretin (TTR)
fragments or proteins with a structure similar to TTR (accession numbers: PDB: 1GKO_A and 2PAB_A),
retinol-binding protein 4 (RBP4) isoform X1 (no information in databases such as UNIPROT), and antibody
fragments. Two proteins, APO-AIV and SP-40,40, were upregulated in group A than in O and C
and in group I versus C, but not in group I versus O. Therefore, we suggest these proteins to be considered
as candidates for the diagnosis of SLE. | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Faculdade de Farmácia. Departamento de Análises Clínicas e Toxicológicas. Belo Horizonte, MG, Brasil. | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Belo Horizonte, MG, Brasil. | pt_BR |
Affilliation | Santa Casa de Belo Horizonte. Belo Horizonte, MG, Brasil. | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Faculdade de Farmácia. Departamento de Análises Clínicas e Toxicológicas. Belo Horizonte, MG, Brasil. | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Belo Horizonte, MG, Brasil. | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Belo Horizonte, MG, Brasil. | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Belo Horizonte, MG, Brasil. | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Belo Horizonte, MG, Brasil. | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Faculdade de Farmácia. Departamento de Análises Clínicas e Toxicológicas. Belo Horizonte, MG, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ. Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ. Brasil. | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Faculdade de Farmácia. Departamento de Análises Clínicas e Toxicológicas. Belo Horizonte, MG, Brasil. | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Faculdade de Farmácia. Departamento de Análises Clínicas e Toxicológicas. Belo Horizonte, MG, Brasil. | pt_BR |
Subject | Systemic lupus erythematosus | pt_BR |
Subject | 2D-DIGE | pt_BR |
Subject | retinol-binding protein 4 isoform X1 | pt_BR |
Subject | SP-40 | pt_BR |
Subject | 40 partial | pt_BR |
Subject | proteomics | pt_BR |
DeCS | Eletroforese em Gel Diferencial Bidimensional | pt_BR |
DeCS | Lúpus Eritematoso Sistêmico | pt_BR |
e-ISSN | 1607-842X | |
Embargo date | 2030-01-01 | |