Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/29937
Title: Wiskott–Aldrich syndrome protein controls antigen-presenting cell-driven CD4+ T-cell motility by regulating adhesion to intercellular adhesion molecule-1
Authors: Lafouresse, Fanny
Almeida, Vinicius Cotta de
Malet-Engra, Gema
Galy, Anne
Valitutti, Salvatore
Dupré, Loïc
Affilliation: INSERM, U1043 / Université Toulouse III Paul-Sabatier. Centre de Physiopathologie de Toulouse Purpan. 3CNRS, U5282, Toulouse, France.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil.
INSERM, U1043 / Université Toulouse III Paul-Sabatier. Centre de Physiopathologie de Toulouse Purpan. 3CNRS, U5282, Toulouse, France.
INSERM, U951. Généthon, Evry, France.
INSERM, U1043 / Université Toulouse III Paul-Sabatier. Centre de Physiopathologie de Toulouse Purpan. 3CNRS, U5282, Toulouse, France.
INSERM, U1043 / Université Toulouse III Paul-Sabatier. Centre de Physiopathologie de Toulouse Purpan. 3CNRS, U5282, Toulouse, France.
Abstract: T-cell scanning for antigen-presenting cells (APC) is a finely tuned process. Whereas non-cognate APC trigger T-cell motility via chemokines and intercellular adhesion molecule-1 (ICAM-1), cognate APC deliver a stop signal resulting from antigen recognition. We tested in vitro the contribution of the actin cytoskeleton regulator Wiskott–Aldrich syndrome protein (WASP) to the scanning activity of primary human CD4+ T cells. WASP knock-down resulted in increased T-cell motility upon encounter with non-cognate dendritic cells or B cells and reduced capacity to stop following antigen recognition. The high motility of WASP-deficient T cells was accompanied by a diminished ability to round up and to stabilize pauses. WASP-deficient T cells migrated in a normal proportion towards CXCL12, CCL19 and CCL21, but displayed an increased adhesion and elongation on ICAM-1. The elongated morphology of WASP-deficient T cells was related to a reduced confinement of high-affinity lymphocyte function-associated antigen 1 to the mid-cell zone. Our data therefore indicate that WASP controls CD4+ T-cell motility upon APC encounter by regulating lymphocyte function-associated antigen 1 spatial distribution.
Keywords: antigen-presenting cell scanning
lymphocyte function-associated antigen 1
T-cell motility
Wiskott–Aldrich syndrome protein
keywords: varredura de células apresentadoras de antígenos
antígeno associado à função linfocitária 1
Motilidade das células T
proteína da síndrome de wiskott-Aldrich
Issue Date: 2012
Publisher: Wiley 12 Months
Citation: LAFOURESSE, Fanny; et al. Wiskott–Aldrich syndrome protein controls antigen-presenting cell-driven CD4+ T-cell motility by regulating adhesion to intercellular adhesion molecule-1. Immunology, v.137, p.183–196, 2012.
DOI: 10.1111/j.1365-2567.2012.03620.x
ISSN: 0019-2805
Copyright: restricted access
Appears in Collections:IOC - Artigos de Periódicos

Files in This Item:
File Description SizeFormat 
viniciuscotta_almeida_etal_IOC_2012.pdf897.03 kBAdobe PDF    Request a copy


FacebookTwitterDeliciousLinkedInGoogle BookmarksBibTex Format mendeley Endnote DiggMySpace

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.