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2030-01-01
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- IOC - Artigos de Periódicos [12448]
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WISKOTT–ALDRICH SYNDROME PROTEIN CONTROLS ANTIGEN-PRESENTING CELL-DRIVEN CD4+ T-CELL MOTILITY BY REGULATING ADHESION TO INTERCELLULAR ADHESION MOLECULE-1
antígeno associado à função linfocitária 1
Motilidade das células T
proteína da síndrome de wiskott-Aldrich
lymphocyte function-associated antigen 1
T-cell motility
Wiskott–Aldrich syndrome protein
Author
Affilliation
INSERM, U1043 / Université Toulouse III Paul-Sabatier. Centre de Physiopathologie de Toulouse Purpan. 3CNRS, U5282, Toulouse, France.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil.
INSERM, U1043 / Université Toulouse III Paul-Sabatier. Centre de Physiopathologie de Toulouse Purpan. 3CNRS, U5282, Toulouse, France.
INSERM, U951. Généthon, Evry, France.
INSERM, U1043 / Université Toulouse III Paul-Sabatier. Centre de Physiopathologie de Toulouse Purpan. 3CNRS, U5282, Toulouse, France.
INSERM, U1043 / Université Toulouse III Paul-Sabatier. Centre de Physiopathologie de Toulouse Purpan. 3CNRS, U5282, Toulouse, France.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil.
INSERM, U1043 / Université Toulouse III Paul-Sabatier. Centre de Physiopathologie de Toulouse Purpan. 3CNRS, U5282, Toulouse, France.
INSERM, U951. Généthon, Evry, France.
INSERM, U1043 / Université Toulouse III Paul-Sabatier. Centre de Physiopathologie de Toulouse Purpan. 3CNRS, U5282, Toulouse, France.
INSERM, U1043 / Université Toulouse III Paul-Sabatier. Centre de Physiopathologie de Toulouse Purpan. 3CNRS, U5282, Toulouse, France.
Abstract
T-cell scanning for antigen-presenting cells (APC) is a finely tuned process.
Whereas non-cognate APC trigger T-cell motility via chemokines
and intercellular adhesion molecule-1 (ICAM-1), cognate APC deliver a
stop signal resulting from antigen recognition. We tested in vitro the contribution
of the actin cytoskeleton regulator Wiskott–Aldrich syndrome
protein (WASP) to the scanning activity of primary human CD4+ T cells.
WASP knock-down resulted in increased T-cell motility upon encounter
with non-cognate dendritic cells or B cells and reduced capacity to stop
following antigen recognition. The high motility of WASP-deficient T cells
was accompanied by a diminished ability to round up and to stabilize
pauses. WASP-deficient T cells migrated in a normal proportion towards
CXCL12, CCL19 and CCL21, but displayed an increased adhesion and
elongation on ICAM-1. The elongated morphology of WASP-deficient
T cells was related to a reduced confinement of high-affinity lymphocyte
function-associated antigen 1 to the mid-cell zone. Our data therefore
indicate that WASP controls CD4+ T-cell motility upon APC
encounter by regulating lymphocyte function-associated antigen 1 spatial
distribution.
Keywords in Portuguese
varredura de células apresentadoras de antígenosantígeno associado à função linfocitária 1
Motilidade das células T
proteína da síndrome de wiskott-Aldrich
Keywords
antigen-presenting cell scanninglymphocyte function-associated antigen 1
T-cell motility
Wiskott–Aldrich syndrome protein
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