Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/30123
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dc.contributor.authorCastro, Andressa da Silva
dc.contributor.authorAlves, Celene Maria de Oliveira Simões
dc.contributor.authorAngeloni, Mariana Bodini
dc.contributor.authorGomes, Angélica Oliveira
dc.contributor.authorBarbosa, Bellisa de Freitas
dc.contributor.authorFranco, Priscila Silva
dc.contributor.authorSilva, Deise Aparecida de Oliveira
dc.contributor.authorMartins Filho, Olindo Assis
dc.contributor.authorMineo, José Roberto
dc.contributor.authorMineo, Tiago Wilson Patriarca
dc.contributor.authorFerro, Eloisa Amália Vieira
dc.date.accessioned2018-11-21T13:27:50Z
dc.date.available2018-11-21T13:27:50Z
dc.date.issued2013
dc.identifier.citationCASTRO, Andressa da Silva et al. Trophoblast cells are able to regulate monocyte activity to control Toxoplasma gondii infection. Placenta, v. 34, n. 3, p. 240-7, 2013
dc.identifier.issn0143-4004
dc.identifier.urihttps://www.arca.fiocruz.br/handle/icict/30123
dc.language.isoeng
dc.publisherElsevier
dc.rightsrestricted access
dc.subject.otherCélula Trofoblástica
dc.subject.otherMonócitos
dc.subject.otherToxoplasma gondii
dc.subject.othercitocinas
dc.titleTrophoblast cells are able to regulate monocyte activity to control Toxoplasma gondii infection
dc.typeArticle
dc.identifier.doi10.1016/j.placenta.2012.12.006
dc.description.abstractenIntroduction: Toxoplasma gondii is an intracellular parasite that causes severe disease when the infection occurs during pregnancy. Trophoblast cells constitute an important maternal–fetal barrier, with monocytes concentrating around them. Thus, interactions between trophoblasts and monocytes are important for maintaining a successful pregnancy, especially in cases of infection. This study aimed to evaluate the role of trophoblast cells (BeWo line) on monocyte (THP-1 line) activity in the presence or absence of T. gondii infection. Methods: THP-1 cells were stimulated with supernatants of BeWo cells, previously infected or not with T. gondii, and then infected with parasites. The supernatant of both cells were collected and analyzed for cytokine production and T. gondii proliferation in THP-1 cells was determined. Results: The results showed that after infection, the pattern of cytokines secreted by THP-1 and BeWo cells was characterized as a pro-inflammatory profile. Furthermore, supernatant of BeWo cells infected or not, was able to change the cytokine profile secreted by infected THP-1 cells, and this supernatant became THP-1 cells more able to control T. gondii proliferation than those that had not been stimulated. Discussion: This effect was associated with secretion of interleukin (IL)-6 by the THP-1 cells and soluble factors secreted by BeWo cells, such as IL-6 and MIF. Conclusion: Together, these results suggest that trophoblast cells are able to modulate monocyte activity, resulting in the control of T. gondii infection and subsequent maintenance of pregnancy.
dc.creator.affilliationUniversidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Laboratório de Histologia e Embriologia. Uberlândia, MG, Brazil
dc.creator.affilliationUniversidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Laboratório de Histologia e Embriologia. Uberlândia, MG, Brazil/Universidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Laboratoro de Imunoparasitologia. Uberlândia, MG, Brazil
dc.creator.affilliationUniversidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Laboratório de Histologia e Embriologia. Uberlândia, MG, Brazil
dc.creator.affilliationUniversidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Laboratório de Histologia e Embriologia. Uberlândia, MG, Brazil
dc.creator.affilliationUniversidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Laboratório de Histologia e Embriologia. Uberlândia, MG, Brazil
dc.creator.affilliationUniversidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Laboratório de Histologia e Embriologia. Uberlândia, MG, Brazil
dc.creator.affilliationUniversidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Laboratoro de Imunoparasitologia. Uberlândia, MG, Brazil
dc.creator.affilliationFundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratorio de Doença de Chagas. Belo Horizonte, MG, Brazil
dc.creator.affilliationUniversidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Laboratoro de Imunoparasitologia. Uberlândia, MG, Brazil
dc.creator.affilliationUniversidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Laboratoro de Imunoparasitologia. Uberlândia, MG, Brazil
dc.creator.affilliationUniversidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Laboratório de Histologia e Embriologia. Uberlândia, MG, Brazil
dc.subject.enTrophoblast cell
dc.subject.enMonocytes
dc.subject.enToxoplasma gondii
dc.subject.enCytokines
Appears in Collections:MG - IRR - Artigos de Periódicos

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