Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/30316
Title: The biological in vitro effect and selectivity of aromatic dicationic compounds on Trypanosoma cruzi
Authors: Silva, Cristiane França da
Silva, Patrícia Bernadino da
Batista, Marcos Meuser
Daliry, Anissa
Tidwell, Richard R.
Soeiro, Maria de Nazaré Correia
Affilliation: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ. Brasil.
University of North Carolina at Chapel Hill. Department of Pathology and Laboratory Medicine. Chapell Hill, NC, USA.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ. Brasil.
Abstract: Trypanosoma cruzi is a parasite that causes Chagas disease, which affects millions of individuals in endemic areas of Latin America. One hundred years after the discovery of Chagas disease, it is still considered a neglected illness because the available drugs are unsatisfactory. Aromatic compounds represent an important class of DNA minor groove-binding ligands that exhibit potent antimicrobial activity. This study focused on the in vitro activity of 10 aromatic dicationic compounds against bloodstream trypomastigotes and intracellular forms of T. cruzi. Our data demonstrated that these compounds display trypanocidal effects against both forms of the parasite and that seven out of the 10 compounds presented higher anti-parasitic activity against intracellular parasites compared with the bloodstream forms. Additional assays to determine the potential toxicity to mammalian cells showed that the majority of the dicationic compounds did not considerably decrease cellular viability. Fluorescent microscopy analysis demonstrated that although all compounds were localised to a greater extent within the kinetoplast than the nucleus, no correlation could be found between compound activity and kDNA accumulation. The present results stimulate further investigations of this class of compounds for the rational design of new chemotherapeutic agents for Chagas disease.
Keywords: aromatic compounds
Trypanosoma cruzi
chemotherapy
Chagas disease
keywords: Doença de Chagas
Quimioterapia
Trypanosoma cruzi
compostos aromáticos
Issue Date: 2010
Publisher: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz
Citation: SILVA, Cristiana França da; et al. The biological in vitro effect and selectivity of aromatic dicationic compounds on Trypanosoma cruzi. Mem Inst Oswaldo Cruz, v.105, n.3, p.239-245, May 2010.
ISSN: 0074-0276
Copyright: open access
Appears in Collections:IOC - Artigos de Periódicos

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