Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/30403
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dc.contributor.authorCoutinho, Joana O P A
dc.contributor.authorSilva, Mariana Pereira dos Santos
dc.contributor.authorMoraes, P.M.
dc.contributor.authorMonteiro, Andrea de Souza
dc.contributor.authorBarcelos, J.C.C.
dc.contributor.authorSiqueira Filho, Ezequias Pessoa de
dc.contributor.authorSantos, Vera Lucia
dc.date.accessioned2018-12-05T16:28:12Z
dc.date.available2018-12-05T16:28:12Z
dc.date.issued2013
dc.identifier.citationCOUTINHO, Joana O P A et al. Demulsifying properties of extracellular products and cells of Pseudomonas aeruginosa MSJ isolated from petroleum-contaminated soil. Bioresour Technol., v. 128, p. 646-654, 2013
dc.identifier.issn0960-8524
dc.identifier.urihttps://www.arca.fiocruz.br/handle/icict/30403
dc.language.isoeng
dc.publisherElsevier Science Pub. Co
dc.rightsrestricted access
dc.subject.otherPseudomonas aeruginosa
dc.subject.otherDemulsificação
dc.subject.otherEmulsões de água em óleo
dc.titleDemulsifying properties of extracellular products and cells of Pseudomonas aeruginosa MSJ isolated from petroleum-contaminated soil
dc.typeArticle
dc.identifier.doi10.1016/j.biortech.2012.09.137
dc.description.abstractenA strain of Pseudomonas aeruginosa isolated from a site contaminated with refined oil products exhibited demulsification capabilities against Tween 80-Span 80 stabilized oil-in-water (O/W), Tween 80-stabilized water-in-oil (W/O) model emulsions (kerosene-water), and an industrial emulsion (Daido Dairoll PA-5A). GC-MS analysis confirmed the presence of fatty acids and carbohydrates in the extracellular biodemulsifier. The demulsifying activity of cells and culture supernatants was favored by growth in media containing 1% diesel oil. There was a correlation between culture age, de-emulsification and cellular hydrophobicity, and highest activities were observed for cells and supernatants from 96-h cultures. Activity increased with addition of up to 60 mg cells or 300 μL supernatant to emulsions. The activity was relatively stable at 20-40 °C and to freezing, but was reduced by 69% by washing the cells with chloroform-methanol-water. This demulsifier has potential for application in biotreatment of emulsified oily wastewaters to promote recovery and/or degradation of oil.
dc.creator.affilliationUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brazil
dc.creator.affilliationUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brazil
dc.creator.affilliationUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brazil
dc.creator.affilliationUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brazil/Universidade Vale do Rio Doce. Faculdade de Ciências da Saúde. Laboratório de Pesquisa em Microbiologia. MG, Brazil
dc.creator.affilliationUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brazil
dc.creator.affilliationFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Química de Produtos Naturais. Belo Horizonte, MG, Brazil
dc.creator.affilliationUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brazil
dc.subject.enPseudomonas aeruginosa
dc.subject.enDemulsification
dc.subject.enWater-in-oil emulsions
Appears in Collections:MG - IRR - Artigos de Periódicos

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