Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/30411
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dc.contributor.authorAngeloni, Mariana Bodini
dc.contributor.authorGuirelli, Pamela Mendonça
dc.contributor.authorFranco, Priscila Silva
dc.contributor.authorBarbosa, Bellisa de Freitas
dc.contributor.authorGomes, Angelica de Oliveira
dc.contributor.authorCastro, Andressa da Silva
dc.contributor.authorSilva, Neide Maria da
dc.contributor.authorMartins Filho, Olindo Assis
dc.contributor.authorMineo, Tiago Wilson Patriarca
dc.contributor.authorSilva, Deise Aparecida de Oliveira
dc.contributor.authorMineo, Jose Roberto
dc.contributor.authorFerro, Eloisa Amália Vieira
dc.date.accessioned2018-12-05T18:04:22Z
dc.date.available2018-12-05T18:04:22Z
dc.date.issued2013
dc.identifier.citationANGELONI, Mariana Bodini et al. Differential apoptosis in BeWo cells after infection with highly (RH) or moderately (ME49) virulent strains of Toxoplasma gondii is related to the cytokine profile secreted, the death receptor Fas expression and phosphorylated ERK1/2 expression. Placenta, v.34, n. 11, p. 973-982, 2013
dc.identifier.issn0143-4004
dc.identifier.urihttps://www.arca.fiocruz.br/handle/icict/30411
dc.language.isoeng
dc.publisherElsevier
dc.rightsrestricted access
dc.subject.otherApoptose
dc.subject.otherCélulas BeWo
dc.subject.otherToxoplasmose congênita
dc.subject.otherToxoplasma gondii
dc.subject.otherCélulas Trofoblásticas
dc.titleDifferential apoptosis in BeWo cells after infection with highly (RH) or moderately (ME49) virulent strains of Toxoplasma gondii is related to the cytokine profile secreted, the death receptor Fas expression and phosphorylated ERK1/2 expression
dc.typeArticle
dc.identifier.doi10.1016/j.placenta.2013.09.005
dc.description.abstractenINTRODUCTION: Alterations of apoptosis are commonly associated with pregnancy complications and abortion. Modulation of apoptosis is a relevant feature of Toxoplasma gondii infection and it is related to parasite strain types. The aim of the present study was to evaluate the possible factors that are involved in the differential apoptosis of BeWo cells infected with distinct T. gondii strain types. METHODS: Human trophoblastic cells (BeWo cell line) were infected with RH or ME49 strains, the cytokine production was measured and the phosphorylation of anti-apoptotic ERK1/2 protein was analyzed. Also, cells were treated with different cytokines, infected with RH or ME49 strain, and analyzed for apoptosis index and Fas/CD95 death receptor expression. RESULTS: ME49-infected BeWo cells exhibited a predominantly pro-inflammatory cytokine profile, whereas cells infected with RH strain had a higher production of anti-inflammatory cytokines. Also, the incidence of apoptosis was higher in ME49-infected cells, which have been treated with pro-inflammatory cytokines compared to cells infected with RH and treated with anti-inflammatory cytokines. Moreover, Fas/CD95 expression was higher in cells infected with either ME49 or RH strain and treated with pro-inflammatory cytokines compared to anti-inflammatory cytokine treatment. The phosphorylation of ERK1/2 protein increased after 24 h of infection only with the RH strain. CONCLUSION: These results suggest that opposing mechanisms of interference in apoptosis of BeWo cells after infection with RH or ME49 strains of T. gondii can be associated with the differential cytokine profile secreted, the Fas/CD95 expression and the phosphorylated ERK1/2 expression.
dc.creator.affilliationUniversidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Laboratório de Histologia e Embriologia. Uberlândia, MG, Brazil
dc.creator.affilliationUniversidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Laboratório de Histologia e Embriologia. Uberlândia, MG, Brazil
dc.creator.affilliationUniversidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Laboratório de Histologia e Embriologia. Uberlândia, MG, Brazil
dc.creator.affilliationUniversidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Laboratório de Histologia e Embriologia. Uberlândia, MG, Brazil
dc.creator.affilliationUniversidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Laboratório de Histologia e Embriologia. Uberlândia, MG, Brazil
dc.creator.affilliationUniversidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Laboratório de Histologia e Embriologia. Uberlândia, MG, Brazil
dc.creator.affilliationUniversidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Laboratório de Imunopatologia. Uberlândia, MG, Brazil
dc.creator.affilliationFundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratório de Doença de Chagas. Belo Horizonte, MG, Brazil
dc.creator.affilliationUniversidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Laboratório de Imunoparasitologia. Uberlândia, MG, Brazil
dc.creator.affilliationUniversidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Laboratório de Imunoparasitologia. Uberlândia, MG, Brazil
dc.creator.affilliationUniversidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Laboratório de Imunoparasitologia. Uberlândia, MG, Brazil
dc.creator.affilliationUniversidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Laboratório de Histologia e Embriologia. Uberlândia, MG, Brazil
dc.subject.enApoptosis
dc.subject.enBeWo cells
dc.subject.enCongenital toxoplasmosis
dc.subject.enToxoplasma gondii
dc.subject.enTrophoblastic cells
Appears in Collections:MG - IRR - Artigos de Periódicos

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