Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/30970
Title: Differential proteomics of the plasma of individuals with sepsis caused by Acinetobacter baumannii
Authors: Soares, Afonso J. C.
Santos, M. F.
Trugilho, M. R. O.
Neves-Ferreira, A. G. C.
Perales, Jonas
Domont, G. B.
Affilliation: Universidade Federal do Rio de Janeiro. Instituto de Química.Unidade de Proteômica. Laboratório de Química Proteica. Rio de Janeiro, RJ, Brasil / Hospital da Força Aérea Brasileira. Rio de Janeiro, RJ, Brasil / Rede Proteômica do Rio de Janeiro. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Instituto de Química.Unidade de Proteômica. Laboratório de Química Proteica. Rio de Janeiro, RJ, Brasil / Rede Proteômica do Rio de Janeiro. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil / Rede Proteômica do Rio de Janeiro. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil / Rede Proteômica do Rio de Janeiro. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil / Rede Proteômica do Rio de Janeiro. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Instituto de Química.Unidade de Proteômica. Laboratório de Química Proteica. Ri de Janeiro, RJ, Brasil / Rede Proteômica do Rio de Janeiro. Rio de Janeiro, RJ, Brasil.
Abstract: This study examines alterations in the plasma proteome in ten adults affected by sepsis caused by Acinetobacter baumannii as compared to paired healthy controls. 2-DE profiles of plasma from patients and paired healthy donors, depleted of the six most abundant proteins, were analysed by the DIGE technique. Protein spot detection and quantification were performed with the Differential In-gel Analysis and Biological Variation Analysis modules of the DeCyder() software. Differentially expressed proteins were identified by mass spectrometry (MALDI-TOF/TOF) after colloidal Coomassie blue staining. Almost 900 spots were detected on a unique 2-D gel by the DIGE technique. A total of 269 protein spots of differential abundance were shown to be statistically significant (2.5-fold) with p values of p< or =0.01 (135 spots) and p< or =0.05 (134 spots) as determined by the t test. Seventy-one spots were submitted to mass spectrometry and about 30% could be successfully identified. This multiplex approach significantly reduced experimental variability, allowing for the confident detection of small differences in protein levels. Results include differentially expressed lipoproteins as well as proteins belonging to inflammatory/coagulation pathways and the kallikrein-kinin system. These data improves the knowledge for future developments in sepsis diagnosis, staging and therapy.
Keywords: Sepsis
Acinetobacter baumannii
Plasma protein depletion
DIGE
Differential proteome
keywords: Acinetobacter baumannii
Proteoma diferencial
Sepse
Depleção de proteína plasmática
Técnica DIGE
Issue Date: 2009
Publisher: Elsevier
Citation: SOARES, Afonso J. C. et al. Differential proteomics of the plasma of individuals with sepsis caused by Acinetobacter baumannii. Journal of Proteomics, v. 73, p. 267-278, 2009.
DOI: 10.1016/j.jprot.2009.09.010
ISSN: 1874-3919
Copyright: restricted access
Appears in Collections:IOC - Artigos de Periódicos

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