Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/31025
Title: A new approach for potential drug target discovery through in silico metabolic pathway analysis using Trypanosoma cruzi genome information
Authors: Ferreira, Marcelo Alves
Gumarães, Ana Carolina Ramos
Capriles, Priscila Vanessa da Silva Zabala
Dardenne, Laurent E.
Degrave, Wim M.
Affilliation: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genômica Funcional e Bioinformática. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genômica Funcional e Bioinformática. Rio de Janeiro, RJ. Brasil.
Ministério da Ciência e Tecnologia. Laboratório Nacional de Computação Científica. Grupo de Modelagem Molecular de Sistemas Biológicos. Petrópolis, RJ, Brasil.
Ministério da Ciência e Tecnologia. Laboratório Nacional de Computação Científica. Grupo de Modelagem Molecular de Sistemas Biológicos. Petrópolis, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genômica Funcional e Bioinformática. Rio de Janeiro, RJ. Brasil.
Abstract: The current drug options for the treatment of chronic Chagas disease have not been sufficient and high hopes have been placed on the use of genomic data from the human parasite Trypanosoma cruzi to identify new drug targets and develop appropriate treatments for both acute and chronic Chagas disease. However, the lack of a complete assembly of the genomic sequence and the presence of many predicted proteins with unknown or unsure functions has hampered our complete view of the parasite’s metabolic pathways. Moreover, pinpointing new drug targets has proven to be more complex than anticipated and has revealed large holes in our understanding of metabolic pathways and their integrated regulation, not only for this parasite, but for many other similar pathogens. Using an in silico comparative study on pathway annotation and searching for analogous and specific enzymes, we have been able to predict a considerable number of additional enzymatic functions in T. cruzi. Here we focus on the energetic pathways, such as glycolysis, the pentose phosphate shunt, the Krebs cycle and lipid metabolism. We point out many enzymes that are analogous to those of the human host, which could be potential new therapeutic targets.
Keywords: Trypanosoma cruzi
Metabolism
Metabolic pathways
Drug target
Analogous enzyme
keywords: Trypanosoma cruzi
Metabolismo
Vias metabólicas
Alvo de drogas
Enzima análoga
Issue Date: 2009
Publisher: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz
Citation: FERREIRA, Marcelo Alves et al. A new approach for potential drug target discovery through in silico metabolic pathway analysis using Trypanosoma cruzi genome information. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 104, n. 8, p. 1100-1110, Dec. 2009.
ISSN: 0074-0276
Copyright: open access
Appears in Collections:IOC - Artigos de Periódicos

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