Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/31047
Title: Early short-term versus prolonged low-dose methylprednisolone therapy in acute lung injury
Authors: Silva, P. L.
Garcia, C. S. N. B.
Maronas, P. A.
Cagido, V. R.
Negri, E. M.
Rodrigues, N. R. Damaceno
Ventura, G. M.
Bozza, Patrícia T.
Zin, W. A.
Capelozi, V. L.
Pelosi, P.
Rocco, P. R. M.
Affilliation: Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Investigação Pulmonar. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Investigação Pulmonar. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Fisiologia Respiratória. Rio de Janeiro, RJ, Brasil.
Universidade de São Paulo. Faculdade de Medicina. Departamento de Patologia. São Paulo, SP, Brasil.
Universidade de São Paulo. Faculdade de Medicina. Departamento de Patologia. São Paulo, SP, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ. Brasil.
Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Fisiologia Respiratória. Rio de Janeiro, RJ, Brasil.
Universidade de São Paulo. Faculdade de Medicina. Departamento de Patologia. São Paulo, SP, Brasil.
University of Insubria. Dept of Ambient, Health and Safety. Varese, Italy.
Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Investigação Pulmonar. Rio de Janeiro, RJ, Brasil.
Abstract: The present study compared the effects of early short-term with prolonged low-dose corticosteroid therapy in acute lung injury (ALI). In total, 120 BALB/c mice were randomly divided into five groups. In the control group, saline was intratracheally (i.t.) instilled. In the ALI group, mice received Escherichia coli lipopolysaccharide (10 mg i.t.). ALI animals were further randomised into four subgroups to receive saline (0.1 mL i.v.) or methylprednisolone (2 mg?kg-1 i.v.) at 6 h, 24 h or daily (for 7 days, beginning at day 1). At 1, 3 and 8 weeks, in vivo and in vitro lung mechanics and histology (light and electron microscopy), collagen and elastic fibre content, cytokines in bronchoalveolar lavage fluid and the expression of matrix metalloproteinase (MMP)-9 and -2 were measured. In vivo (static elastance and viscoelastic pressure) and in vitro (tissue elastance and resistance) lung mechanics, alveolar collapse, cell infiltration, collagen and elastic fibre content and the expression of MMP-9 and MMP-2 were increased in ALI at 1 week. Methylprednisolone led to a complete resolution of lung mechanics, avoided fibroelastogenesis and the increase in the expression of MMP-9 and MMP-2 independent of steroid treatment design. Thus, early short-term, low-dose methylprednisolone is as effective as prolonged therapy in acute lung injury.
Keywords: Acute lung injury
Collagen
Corticosteroid
Elastance
Lung histology
Matrix metalloproteinase
keywords: Lesão pulmonar aguda
Matriz metaloproteinase
Colágeno
Corticosteróide
Elastância
Histologia pulmonar
Issue Date: 2009
Publisher: European Respiratory Society
Citation: SILVA, P. L. et al. Early short-term versus prolonged low-dose methylprednisolone therapy in acute lung injury. Eur. Resp, J., v. 33, n. 3, p. 634-645, 2009.
ISSN: 0903-1936
Copyright: restricted access
Appears in Collections:IOC - Artigos de Periódicos

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