Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/31086
Title: Evaluation of an adsorption-elution method for detection of astrovirus and norovirus in environmental waters
Authors: Victoria, Matías
Guimarães, Flávia
Fumian, Tulio
Vieira, Carmen
Leite, José Paulo
Miagostovich, Marize
Affilliation: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada. Rio de Janeiro, RJ, Brasil.
Abstract: Human astroviruses (HAstV) and noroviruses (NoV) are shed frequently at high concentrations and persist for long periods in the environment, raising a significant health risk of water-related gastroenteritis. The aim of this study was to evaluate an adsorption-elution method with an HA (mixed cellulose esters) negatively charged membrane to determine the best recovery of HAstV and NoV from different environmental waters. As the presence of MgCl(2) affects viral adsorption onto the membrane, three different MgCl(2) concentrations were evaluated. The best recovery of both NoV and HAstV from mineral and river water samples was between 18% and 64%, while recovery from tap water and sea water samples was between 3% and 14%. These results suggest that detection and recovery of each enteric virus with this adsorption-elution method requires a specific MgCl(2) concentration and depends on the source of environmental water tested. The combination of the HA negatively charged membrane to concentrate viruses with quantitative PCR detection allows for the identification of gastroenteritis viruses implicated in acute outbreaks of gastroenteritis.
Keywords: Virus recovery
Astrovirus
Norovirus
Environmental water samples
keywords: Recuperação de vírus
Norovirus
Astrovirus
Amostras de água ambiental
Issue Date: 2009
Publisher: Elsevier
Citation: VICTORIA, Matias; et al. Evaluation of an adsorption–elution method for detection of astrovirus and norovirus in environmental waters. Journal of Virological Methods, v.156, p.73-76, 2009.
DOI: 10.1016/j.jviromet.2008.11.003
ISSN: 0166-0934
Copyright: restricted access
Appears in Collections:IOC - Artigos de Periódicos

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