Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/31115
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dc.contributor.authorValente, Richard H.
dc.contributor.authorJunqueira, Magno
dc.contributor.authorFerreira, Ana Gisele C. Neves
dc.contributor.authorChapeaurouge, Alex
dc.contributor.authorTrugilho, Monique R. O.
dc.contributor.authorLeón, Ileana R.
dc.contributor.authorRocha, Surza L. G.
dc.contributor.authorPerales, Jonas
dc.contributor.authorMúltipla autoria - ver em Notas
dc.date.accessioned2019-01-17T14:57:38Z
dc.date.available2019-01-17T14:57:38Z
dc.date.issued2009
dc.identifier.citationVALENTE, Richard H. et al. Bothrops insularis venomics: A proteomic analysis supported by transcriptomic-generated sequence data. Journal of Proteomics, v. 72, p. 241-255, 2009.
dc.identifier.issn1874-3919
dc.identifier.urihttps://www.arca.fiocruz.br/handle/icict/31115
dc.descriptionAUTHORS - Richard H. Valentea,e,⁎, Patrícia R. Guimarãesc,e, Magno Junqueiraa,e, Ana Gisele C. Neves-Ferreiraa,e, Márcia R. Soaresb,f, Alex Chapeaurougea,e, Monique R.O. Trugilhoa,e, Ileana R. Leóna,e, Surza L.G. Rochaa,b,e, Ana Lucia Oliveira-Carvalhoc,e, Luciana S. Wermelingerc,e, Denis L.S. Dutrac,e, Luciana I. Leãod,g, Inácio L.M. Junqueira-de-Azevedod,g, Paulo L. Hod, Russolina B. Zingalic,e, Jonas Peralesa,e, Gilberto B. Domontb,e,⁎ - AFFILIATIONS- aOswaldo Cruz Foundation, IOC, Laboratory of Toxinology, Rio de Janeiro, Brazil bFederal University of Rio de Janeiro, Department of Biochemistry, Institute of Chemistry, Rio de Janeiro, RJ, Brazil c Federal University of Rio de Janeiro, Instituto de Bioquímica Médica, Rio de Janeiro, RJ, Brazil dButantan Institute, São Paulo, SP, Brazil eRio de Janeiro Proteomics Network, Brazil fNational Laboratory of Synchrotron Light, Campinas, SP, Brazil gUniversity of São Paulo, Bioscience Institute, SP, Brazil.
dc.language.isoeng
dc.publisherElsevier
dc.rightsrestricted access
dc.subject.otherBothrops insularis
dc.subject.otherVenenos de Serpentes
dc.subject.otherProteômica
dc.subject.otherTranscriptômica
dc.titleBothrops insularis venomics: a proteomic analysis supported by transcriptomic-generated sequence data
dc.typeArticle
dc.identifier.doi10.1016/j.jprot.2009.01.001
dc.description.abstractenA joint transcriptomic and proteomic approach employing two-dimensional electrophoresis, liquid chromatography and mass spectrometry was carried out to identify peptides and proteins expressed by the venom gland of the snake Bothrops insularis, an endemic species of Queimada Grande Island, Brazil. Four protein families were mainly represented in processed spots, namely metalloproteinase, serine proteinase, phospholipase A(2) and lectin. Other represented families were growth factors, the developmental protein G10, a disintegrin and putative novel bradykinin-potentiating peptides. The enzymes were present in several isoforms. Most of the experimental data agreed with predicted values for isoelectric point and M(r) of proteins found in the transcriptome of the venom gland. The results also support the existence of posttranslational modifications and of proteolytic processing of precursor molecules which could lead to diverse multifunctional proteins. This study provides a preliminary reference map for proteins and peptides present in Bothrops insularis whole venom establishing the basis for comparative studies of other venom proteomes which could help the search for new drugs and the improvement of venom therapeutics. Altogether, our data point to the influence of transcriptional and post-translational events on the final venom composition and stress the need for a multivariate approach to snake venomics studies.
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil / Rede Proteômica do Rio de Janeiro. Rio de Janeiro, RJ, Brasil.
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil / Rede Proteômica do Rio de Janeiro. Rio de Janeiro, RJ, Brasil.
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil / Rede Proteômica do Rio de Janeiro. Rio de Janeiro, RJ, Brasil.
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil / Rede Proteômica do Rio de Janeiro. Rio de Janeiro, RJ, Brasil.
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil / Rede Proteômica do Rio de Janeiro. Rio de Janeiro, RJ, Brasil.
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil / Rede Proteômica do Rio de Janeiro. Rio de Janeiro, RJ, Brasil.
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Química. Departamento de Bioquímica. Rio de Janeiro, RJ, Brasil / Rede Proteômica do Rio de Janeiro. Rio de Janeiro, RJ, Brasil.
dc.creator.affilliationundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil / Rede Proteômica do Rio de Janeiro. Rio de Janeiro, RJ, Brasil.
dc.creator.affilliationMúltipla autoria - ver Notas
dc.subject.enBothrops insularis
dc.subject.enProteomics
dc.subject.enTranscriptomics
dc.subject.enSnake venom
dc.identifier.eissn1876-7737
Appears in Collections:IOC - Artigos de Periódicos

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