Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/31170
Title: Steady-state level of bone morphogenetic protein-15 in goat ovaries and its influence on in vitro development and survival of preantral follicles
Authors: Celestino, J. J. H.
Lima-Verde, I. B.
Bruno, J. B.
Matos, M. H. T.
Chaves, R. N.
Saraiva, M. V. A.
Silva, C. M. G.
Faustino, L. R.
Rossetto, R.
Lopes, C. A. P.
Donato, M. A. M.
Peixoto, C. A.
Campello, C. C.
Silva, J. R. V.
Figueiredo, J. R.
Abstract: This study investigates steady-state level of bone morphogenetic protein-15 (BMP-15) mRNA in caprine follicles, and the effects of BMP-15 on in vitro development of preantral follicles. Ovarian fragments were cultured for one or seven days in Minimal Essential Medium (MEM(+)) with BMP-15 (0, 1, 10, 50, 100 or 200 ng/mL), and further analyzed by histology, transmission electron and fluorescent microscopy. BMP-15 mRNA in secondary follicles was higher than in primordial and primary follicles. After seven days, 10, 50 or 100 ng/mL of BMP-15 maintained the percentage of normal follicles similar to the control (non-cultured), and increased the oocyte and follicle diameters when compared to the control and MEM(+). BMP-15 at 100 ng/mL increased the secondary follicles and maintained their ultrastructural integrity. In conclusion, the BMP-15 mRNAs were detected in all follicular categories. BMP-15 (100 ng/mL) maintained the integrity and promoted the growth of caprine preantral follicles cultured for seven days.
Keywords: Caprine
Preantral follicles
BMP-15
Activation
Growth
DeCS: Animais
Proteína Morfogenética Óssea 15 / genética
Morfogenética Óssea 15 / metabolismo
Proteína Morfogenética Óssea 15 / farmacologia
Sobrevivência Celular / efeitos de drogas
Fêmea
Caprinos / crescimento e desenvolvimento
Microscopia Eletrônica, Transmissão
Oócitos / efeitos de drogas
Oócitos / crescimento e desenvolvimento
Folículo Ovariano / anatomia & histologia
Folículo Ovariano / efeitos de drogas
Folículo Ovariano / crescimento e desenvolvimento
Folículo Ovariano / metabolismo
Técnicas de cultura de tecidos
Transcrição Genética
Issue Date: 2011
Citation: CELESTINO, J. J. H. et al. Steady-state level of bone morphogenetic protein-15 in goat ovaries and its influence on in vitro development and survival of preantral follicles. Molecular and Cellular Endocrinology, v. 338, n. 1, p. 1–9, 16 maio 2011.
Description: Faculty of Veterinary Medicine, LAMOFOPA, PPGCV, State University of Ceara, Fortaleza, CE, Brazil Institute for Research and Technology, Tiradentes University, Aracaju, SE, Brazil Nucleus of Biotechnology Applied to Ovarian Follicle Development, Federal University of São Francisco Valley, Petrolina, PE, Brazil Laboratory of Ultrastructure, CPqAM/Fiocruz, Federal University of Pernambuco, Recife, PE, Brazil Biotecnology Nucleus of Sobral (NUBIS), Federal University of Ceara, Sobral, CE, Brazil
DOI: 10.1016/j.mce.2011.02.007
ISSN: 1872-8057
Copyright: restricted access
Appears in Collections:PE - IAM - Artigos de Periódicos



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