Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/31307
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dc.contributor.authorLima, Emilly Juliana Sales Pereira de
dc.contributor.authorAlves, Rafaela G
dc.contributor.authorD'Elia, Gigliola Mayara Ayres
dc.contributor.authorAnunciação, Talita Andrade da
dc.contributor.authorSilva, Valdenizia Rodrigues
dc.contributor.authorSantos, Luciano de S
dc.contributor.authorSoares, Milena Botelho Pereira
dc.contributor.authorCardozo, Nállarett Marina Dávila
dc.contributor.authorCosta, Emmanoel Vilaça
dc.contributor.authorSilva, Felipe M A da
dc.contributor.authorKoolen, Hector Henrique Ferreira
dc.contributor.authorBezerra, Daniel Pereira
dc.date.accessioned2019-01-24T17:56:37Z
dc.date.available2019-01-24T17:56:37Z
dc.date.issued2018
dc.identifier.citationLIMA, E. J. S. P. et al. Antitumor Effect of the Essential Oil from the Leaves of Crotonmatourensis Aubl. (Euphorbiaceae). Molecules, v. 23, p. 2974, 2018.
dc.identifier.issn1420-3049
dc.identifier.urihttps://www.arca.fiocruz.br/handle/icict/31307
dc.description.sponsorshipBrazilian agencies Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Fundação de Amparo à Pesquisa do Estado do Amazonas (FAPEAM), and Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB).
dc.language.isoeng
dc.publisherMDPI
dc.rightsopen access
dc.subject.otherCroton matourensis
dc.subject.otherCroton lanjouwensis
dc.subject.otherAntitumoral
dc.subject.otherApoptose
dc.subject.otherHepG2
dc.titleAntitumor Effect of the Essential Oil from the Leaves of Crotonmatourensis Aubl. (Euphorbiaceae)
dc.typeArticle
dc.identifier.doi10.3390/molecules23112974
dc.description.abstractenCroton matourensis Aubl. (synonym Croton lanjouwensis Jabl.), popularly known as "orelha de burro", "maravuvuia", and/or "sangrad'água", is a medicinal plant used in Brazilian folk medicine as a depurative and in the treatment of infections, fractures, and colds. In this work, we investigated the chemical composition and in vitro cytotoxic and in vivo antitumor effects of the essential oil (EO) from the leaves of C. matourensis collected from the Amazon rainforest. The EO was obtained by hydrodistillation using a Clevenger-type apparatus and characterized qualitatively and quantitatively by gas chromatography coupled to mass spectrometry (GC⁻MS) and gas chromatography with flame ionization detection (GC⁻FID), respectively. In vitro cytotoxicity of the EO was assessed in cancer cell lines (MCF-7, HCT116, HepG2, and HL-60) and the non-cancer cell line (MRC-5) using the Alamar blue assay. Furthermore, annexin V-FITC/PI staining and the cell cycle distribution were evaluated with EO-treated HepG2 cells by flow cytometry. In vivo efficacy of the EO (40 and 80 mg/kg/day) was demonstrated in C.B-17 severe combined immunodeficient (SCID) mice with HepG2 cell xenografts. The EO included β-caryophyllene, thunbergol, cembrene, p-cymene, and β-elemene as major constituents. The EO exhibited promising cytotoxicity and was able to cause phosphatidylserine externalization and DNA fragmentation without loss of the cell membrane integrity in HepG2 cells. In vivo tumor mass inhibition rates of the EO were 34.6% to 55.9%. Altogether, these data indicate the anticancer potential effect of C. matourensis.
dc.creator.affilliationAmazonas State University. Metabolomics and Mass Spectrometry Research Group. Manaus, AM, Brazil.
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.
dc.creator.affilliationAmazonas State University. Metabolomics and Mass Spectrometry Research Group. Manaus, AM, Brazil.
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Center of Biotechnology and Cell Therapy. Salvador, BA, Brazil.
dc.creator.affilliationAmazonia Museum. Manaus, AM, Brazil.
dc.creator.affilliationFederal University of Amazonas. Department of Chemistry. Manaus, AM, Brazil.
dc.creator.affilliationFederal University of Amazonas. Department of Chemistry. Manaus, AM, Brazil.
dc.creator.affilliationAmazonas State University. Metabolomics and Mass Spectrometry Research Group. Manaus, AM, Brazil.
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.
dc.subject.enCroton matourensis
dc.subject.enCroton lanjouwensis
dc.subject.enAntitumor
dc.subject.enApoptosis
dc.subject.enHepG2
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