Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/31424
Title: Assessing hepatitis B immunity using dried blood spot samples from HIV+ individuals
Authors: Flores, Geane Lopes
Cruz, Helena Medina
Miguel, Juliana Custódio
Potsch, Denise Vigo
Pilotto, José Henrique
Lewis-Ximenez, Lia Laura
Lampe, Elisabeth
Villar, Livia Melo
Affilliation: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Hospital Universitário Clementino Fraga Filho. Ambulatório de Doenças Infecciosas. Rio de Janeiro, RJ, Brasil.
Hospital Geral de Nova Iguaçu. Departamento de Doenças Sexualmente Transmissíveis. Nova Iguaçu, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Molecular. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil.
Abstract: This study aims to evaluate the utility of an optimized enzyme immunoassay (EIA) to detect and quantify antibodies against hepatitis B surface antibody (anti-HBs) in dried blood spots (DBSs) within the context of human immunodeficiency virus (HIV) status. Serum and DBS samples were obtained from 56 HIV+ and 99 HIV- patients and subjected to EIA for the detection of anti-HBs, where sample volume and cut off value were modified for DBS testing. Sensitivities of anti-HBs detection in DBS were 79.8% and 76.8% in HIV- and HIV+ subjects, respectively. Concordant results for anti-HBs in serum and DBS presented high mean CD8 cell counts, HIV viral load and optical density (OD) values of anti-HBs. Anti-HBs titers were significantly higher in serum, whether or not anti-HBs titers were detected in DBS. It was possible to detect anti-HBs in DBS as low as 17.4 and 27.3 IU/mL among HIV+ and HIV- subjects, respectively. In conclusion, DBS can be used to detect and quantify anti-HBs in HIV-infected individuals, which could increase access to diagnosis and vaccination.
Keywords: Diagnosis
Dried blood spot
Hepatitis B
Immunodeficiency virus
Immunity
keywords: Hepatite B
Diagnóstico
Imunidade
Vírus da imunodeficiência
Mmancha de sangue seco
Issue Date: 2018
Publisher: Wiley
Citation: FLORES, Geane Lopes et al. Assessing hepatitisBimmunity using dried blood spot samples from HIV+ individuals. Journal of Medical Virology, v. 90, p. 1863-1867, 2018.
DOI: 10.1002/jmv.25275
ISSN: 0146-6615
Copyright: restricted access
Appears in Collections:IOC - Artigos de Periódicos

Files in This Item:
File Description SizeFormat 
geanelopes_flores_etal_IOC_2018.pdf516.84 kBAdobe PDF    Request a copy


FacebookTwitterDeliciousLinkedInGoogle BookmarksBibTex Format mendeley Endnote DiggMySpace

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.