Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/31424
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dc.contributor.authorFlores, Geane Lopes
dc.contributor.authorCruz, Helena Medina
dc.contributor.authorMiguel, Juliana Custódio
dc.contributor.authorPotsch, Denise Vigo
dc.contributor.authorPilotto, José Henrique
dc.contributor.authorLewis-Ximenez, Lia Laura
dc.contributor.authorLampe, Elisabeth
dc.contributor.authorVillar, Livia Melo
dc.date.accessioned2019-02-05T14:05:26Z
dc.date.available2019-02-05T14:05:26Z
dc.date.issued2018
dc.identifier.citationFLORES, Geane Lopes et al. Assessing hepatitisBimmunity using dried blood spot samples from HIV+ individuals. Journal of Medical Virology, v. 90, p. 1863-1867, 2018.
dc.identifier.issn0146-6615
dc.identifier.urihttps://www.arca.fiocruz.br/handle/icict/31424
dc.language.isoeng
dc.publisherWiley
dc.rightsrestricted access
dc.subject.otherHepatite B
dc.subject.otherDiagnóstico
dc.subject.otherImunidade
dc.subject.otherVírus da imunodeficiência
dc.subject.otherMmancha de sangue seco
dc.titleAssessing hepatitis B immunity using dried blood spot samples from HIV+ individuals
dc.typeArticle
dc.identifier.doi10.1002/jmv.25275
dc.description.abstractenThis study aims to evaluate the utility of an optimized enzyme immunoassay (EIA) to detect and quantify antibodies against hepatitis B surface antibody (anti-HBs) in dried blood spots (DBSs) within the context of human immunodeficiency virus (HIV) status. Serum and DBS samples were obtained from 56 HIV+ and 99 HIV- patients and subjected to EIA for the detection of anti-HBs, where sample volume and cut off value were modified for DBS testing. Sensitivities of anti-HBs detection in DBS were 79.8% and 76.8% in HIV- and HIV+ subjects, respectively. Concordant results for anti-HBs in serum and DBS presented high mean CD8 cell counts, HIV viral load and optical density (OD) values of anti-HBs. Anti-HBs titers were significantly higher in serum, whether or not anti-HBs titers were detected in DBS. It was possible to detect anti-HBs in DBS as low as 17.4 and 27.3 IU/mL among HIV+ and HIV- subjects, respectively. In conclusion, DBS can be used to detect and quantify anti-HBs in HIV-infected individuals, which could increase access to diagnosis and vaccination.
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil.
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil.
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil.
dc.creator.affilliationUniversidade Federal do Rio de Janeiro. Hospital Universitário Clementino Fraga Filho. Ambulatório de Doenças Infecciosas. Rio de Janeiro, RJ, Brasil.
dc.creator.affilliationHospital Geral de Nova Iguaçu. Departamento de Doenças Sexualmente Transmissíveis. Nova Iguaçu, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Molecular. Rio de Janeiro, RJ, Brasil.
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil.
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil.
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil.
dc.subject.enDiagnosis
dc.subject.enDried blood spot
dc.subject.enHepatitis B
dc.subject.enImmunodeficiency virus
dc.subject.enImmunity
dc.identifier.eissn1096-9071
Appears in Collections:IOC - Artigos de Periódicos

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