Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/31444
Title: Experimental hyalohyphomycosis by Purpureocillium lilacinum: outcome of the iInfection in C57BL/6 murine models
Authors: Sequeira, Danielly C. M. de
Menezes, Rodrigo C.
Oliveira, Manoel M. E.
Antas, Paulo R. Z.
De Luca, Paula M.
Oliveira-Ferreira, Joseli de
Borba, Cintia de Moraes
Affilliation: Oswaldo Cruz Foundation. Oswaldo Cruz Institute. Laboratory of Taxonomy, Biochemistry and Bioprospecting of Fungi. Rio de Janeiro, RJ, Brazil / Oswaldo Cruz Foundation. Oswaldo Cruz Institute. Laboratory of Immunoparasitology. Rio de Janeiro, RJ, Brazil.
Oswaldo Cruz Foundation. Evandro Chagas National Institute of Infectology Laboratory of Clinical Research in Dermatozoonosis. Rio de Janeiro, RJ, Brazil.
Oswaldo Cruz Foundation. Evandro Chagas National Institute of Infectology. Laboratory of Mycology. Rio de Janeiro, RJ, Brazil.
Oswaldo Cruz Foundation. Oswaldo Cruz Institute. Laboratory of Clinical Immunology. Rio de Janeiro, RJ, Brazil.
Oswaldo Cruz Foundation. Oswaldo Cruz Institute. Laboratory of Immunoparasitology. Rio de Janeiro, RJ, Brazil.
Oswaldo Cruz Foundation. Oswaldo Cruz Institute. Laboratory of Immunoparasitology. Rio de Janeiro, RJ, Brazil.
Oswaldo Cruz Foundation. Oswaldo Cruz Institute. Laboratory of Taxonomy, Biochemistry and Bioprospecting of Fungi. Rio de Janeiro, RJ, Brazil.
Abstract: Purpureocillium lilacinum is a filamentous, hyaline fungus considered an emerging pathogen in humans. The aim of our study was to evaluate the outcome of hyalohyphomycosis in C57BL/6 murine models inoculated with two clinical P. lilacinum isolates (S1 and S2). Each isolate was inoculated in mice randomly distributed in immunocompetent (CPT) and immunosuppressed (SPS) groups. Mice were evaluated at day 7, 21, and 45 after inoculation for histopathological analysis, recovery of fungal cells, and immunological studies. Histological analysis showed scarce conidia-like structures in lung tissue from CPT mice and a lot of fungal cells in SPS mice inoculated with S2 compared to mice inoculated with S1. The maximum recovery of fungal cells was seen in CPT mice inoculated with both isolates at day 7, but with mean significantly higher in those inoculated with S2 isolate. Phenotypical characterization of T cells showed TCD8+ lymphocytes predominance over TCD4+ in immunosuppressed mice infected and control groups. We also observed higher percentages of the central and effector memory/effector phenotype in CPT mice infected with S2 strain, especially in TCD8+ in the initial period of infection. Regulatory T cells showed higher percentages in immunosuppressed, predominantly after the acute phase. Our results showed that the P. lilacinum is a fungus capable to cause damages in competent and immunosuppressed experimental hosts. Furthermore, S2 isolate seems to cause more damage to the experimental host and it was possible to identify different cellular subsets involved in the mice immune response.
Keywords: Purpureocillium lilacinum
Hyalohyphomycosis
Experimental model
Immune response
Immunosuppression
Issue Date: 2017
Publisher: Frontiers Media
Citation: SEQUEIRA, Danielly C. M. de et al. Experimental hyalohyphomycosis by Purpureocillium lilacinum: outcome of the Infection in C57BL/6 murine models. Frontiers in Microbiology, v. 8, p. 1-13, 2017.
DOI: 10.3389/fmicb.2017.01617
ISSN: 1664-302X
Copyright: open access
Appears in Collections:IOC - Artigos de Periódicos
INI - Artigos de Periódicos

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