Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/31524
Title: Mansonella ozzardi mitogenome and pseudogene characterisation provides new perspectives on filarial parasite systematics and CO-1 barcoding
Authors: Crainey, James Lee
Marín, Michel Abanto
Silva, Túllio Romão Ribeiro da
Medeiros, Jansen Fernandes de
Pessoa, Felipe Arley Costa
Santos, Yago Vinícius
Vicente, Ana Carolina Paulo
Luz, Sérgio Luiz Bessa
Affilliation: Fundação Oswaldo Cruz. Instituto Leônidas e Maria Deane. Manaus, AM, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genética Molecular de Microrganismos. Rio de Janeiro, RJ, Brasil / University of La Frontera. Scientific and Technological Bioresource Nucleus. Temuco, Chile.
Fundação Oswaldo Cruz. Instituto Leônidas e Maria Deane. Manaus, AM, Brasil.
Fundação Oswaldo Cruz. Laboratório de Entomologia. Porto Velho, RO, Brasil.
Fundação Oswaldo Cruz. Instituto Leônidas e Maria Deane. Manaus, AM, Brasil.
Fundação Oswaldo Cruz. Instituto Leônidas e Maria Deane. Manaus, AM, Brasil / Programa de Pós-Graduação em Biologia da Interação Patógeno Hospedeiro. Manaus, AM, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genética Molecular de Microrganismos. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Leônidas e Maria Deane. Manaus, AM, Brasil.
Abstract: Despite the broad distribution of M. ozzardi in Latin America and the Caribbean, there is still very little DNA sequence data available to study this neglected parasite's epidemiology. Mitochondrial DNA (mtDNA) sequences, especially the cytochrome oxidase (CO1) gene's barcoding region, have been targeted successfully for filarial diagnostics and for epidemiological, ecological and evolutionary studies. MtDNA-based studies can, however, be compromised by unrecognised mitochondrial pseudogenes, such as Numts. Here, we have used shot-gun Illumina-HiSeq sequencing to recover the first complete Mansonella genus mitogenome and to identify several mitochondrial-origin pseudogenes. Mitogenome phylogenetic analysis placed M. ozzardi in the Onchocercidae "ONC5" clade and suggested that Mansonella parasites are more closely related to Wuchereria and Brugia genera parasites than they are to Loa genus parasites. DNA sequence alignments, BLAST searches and conceptual translations have been used to compliment phylogenetic analysis showing that M. ozzardi from the Amazon and Caribbean regions are near-identical and that previously reported Peruvian M. ozzardi CO1 reference sequences are probably of pseudogene origin. In addition to adding a much-needed resource to the Mansonella genus's molecular tool-kit and providing evidence that some M. ozzardi CO1 sequence deposits are pseudogenes, our results suggest that all Neotropical M. ozzardi parasites are closely related.
Keywords: Mansonella ozzardi
Parasites
Pseudogene
Metagenome
CO-1 barcoding
keywords: Mansonella ozzardi
Parasitas
Pseudogenes
Metagenoma
CO-1 código de barras
Issue Date: 2018
Publisher: Nature Research
Citation: CRAINEY, James Lee et al. Mansonella ozzardi mitogenome and pseudogene characterisation provides new perspectives on filarial parasite systematics and CO-1 barcoding. Scientific Reports, v. 8, n. 6158, p.1-11, Apr. 2018.
DOI: 10.1038/s41598-018-24382-3
ISSN: 2045-2322
Copyright: open access
Appears in Collections:AM - ILMD - Artigos de Periódicos
IOC - Artigos de Periódicos

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