Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/31539
Full metadata record
DC FieldValue
dc.contributor.authorLourenço, Maria Cristina
dc.contributor.authorGrinsztejn, Beatriz
dc.contributor.authorFandinho-Montes, F. C.
dc.contributor.authorda Silva, M. G.
dc.contributor.authorSaad, M. H.
dc.contributor.authorFonseca, L. S.
dc.date.accessioned2019-02-11T13:43:36Z
dc.date.available2019-02-11T13:43:36Z
dc.date.issued2000
dc.identifier.citationLOURENÇO, Maria Cristina S. et al. Genotypic patterns of multiple isolates of M. tuberculosis from tuberculous HIV patients. Tropical Medicine and International Health, v. 5, n. 7, p. 488–494, 2000.
dc.identifier.issn1360-2276
dc.identifier.urihttps://www.arca.fiocruz.br/handle/icict/31539
dc.language.isoeng
dc.publisherBlackwell Science Ltd
dc.rightsopen access
dc.titleGenotypic patterns of multiple isolates of M. tuberculosis from tuberculous HIV patients
dc.typeArticle
dc.identifier.doi10.1046/j.1365-3156.2000.00583.x
dc.description.abstractenWe investigated whether the recurrence of tuberculosis in HIV-infected patients is due to an exogenous reinfection or relapses after antituberculosis chemotherapy. We reviewed clinical information on 32 patients at a Rio de Janeiro hospital from whom multiple Mycobacterium tuberculosis isolates were taken. All isolates were analysed by DRE-PCR fingerprinting technique, and those with identical DRE-PCR patterns were analysed by the RFLP method. Twenty patients had M. tuberculosis simultaneously isolated from different organs. These patients and nine others with sequential positive cultures after 2 months of therapy showed stable DRE-PCR and RFLP patterns. One patient's isolate became resistant to isoniazid, but the molecular pattern remained unchanged despite the development of drug resistance. In three patients, the DRE-PCR patterns of the isolates changed dramatically. Clinical and microbiological evidence was consistent with active tuberculosis caused by a new strain of M. tuberculosis. The exogenous reinfection of the three patients was not due to an outbreak, but the isolates from each patient showed unique patterns.
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Bacteriologia e Bioensaios. Rio de Janeiro, RJ, Brasil.
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em DST/AIDS. Rio de Janeiro, RJ, Brasil.
dc.creator.affilliationFundação Oswaldo Cruz. Instituto de Desenvolvimento Tecnológico e Produtos Imunobiológicos. Rio de Janeiro, RJ, Brasil.
dc.creator.affilliationUniversidade Federal do Rio de Janeiro. Instituto de Microbiologia. Rio de Janeiro, RJ, Brasil.
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.
dc.creator.affilliationUniversidade Federal do Rio de Janeiro. Instituto de Microbiologia. Rio de Janeiro, RJ, Brasil.
dc.subject.enTuberculosis
dc.subject.enHIV coinfection
dc.subject.enMultiple isolates
dc.subject.enGenotypic patterns
Appears in Collections:INI - Artigos de Periódicos

Files in This Item:
File Description SizeFormat 
Genotypic patterns_Beatriz_Grinsztejn_INI_Lapclin-AIDS_2000.pdf3.47 MBAdobe PDF    Request a copy


FacebookTwitterDeliciousLinkedInGoogle BookmarksBibTex Format mendeley Endnote DiggMySpace

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.