Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/31540
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dc.contributor.authorGonçalves, Marcelo Luiz Carvalho
dc.contributor.authorSilva, Valmir Laurentino
dc.contributor.authorAndrade, Carlos Mauricio de
dc.contributor.authorReinhard, Karl Jan
dc.contributor.authorRocha, Gino Chaves da
dc.contributor.authorBailly, Matthieu Le
dc.contributor.authorBouchet, Françoise
dc.contributor.authorSilva, Luiz Fernando Rocha Ferreira da
dc.contributor.authorAraújo, Adauto José Goncalves de
dc.date.accessioned2019-02-11T13:58:15Z
dc.date.available2019-02-11T13:58:15Z
dc.date.issued2004
dc.identifier.citationGONÇALVES, Marcelo Luiz Carvalho et al. Amoebiasis distribution in the past: first steps using an immunoassay technique. Transactions of The Royal Society of Tropical Medicine and Hygiene, v. 98, n. 2, p. 1-4, 2004.
dc.identifier.issn0035-9203
dc.identifier.urihttps://www.arca.fiocruz.br/handle/icict/31540
dc.language.isoeng
dc.publisherTransactions of The Royal Society of Tropical Medicine and Hygiene
dc.rightsopen access
dc.titleAmoebiasis distribution in the past: first steps using a immunoassay technique
dc.typeArticle
dc.description.abstractenThe identification of parasites in ancient human faeces is compromised by differential preservation of identifiable parasite structures. However, protein molecules can survive the damage of the environment and can be detected even after centuries. In this paper it is shown that is possible to detect copro-antigen of Entamoeba histolytica in historic and prehistoric human faecal remains, using a commercially available enzyme immunoassay (ELISA) kit. The kit uses monoclonal antibody-peroxidase conjugate specific for E. histolytica adhesin. A total of 90 specimens of desiccated faeces found in mummies and ancient organic sediment from South America, North America, Africa, and Europe were examined. The ELISA detected 20 positive samples, dated to about 5300 years before present to the 19th Century ad. The positive samples are from archaeological sites in Argentina, USA, France, Belgium, and Switzerland. The detection of protozoan antigen using immunoassays is a reliable tool for the studies of intestinal parasites in the past.
dc.creator.affilliationFundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sergio Arouca. Rio de Janeiro, RJ, Brasil.
dc.creator.affilliationFundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sergio Arouca. Rio de Janeiro, RJ, Brasil.
dc.creator.affilliationFundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sergio Arouca. Rio de Janeiro, RJ, Brasil.
dc.creator.affilliationUniversity of Nebraska. School of Natural Resource Sciences. Lincoln, NE, USA.
dc.creator.affilliationFundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sergio Arouca. Rio de Janeiro, RJ, Brasil / Universite de Reims. U. F. R. de Pharmacie. Laboratoire de Paleoparasitologie. Reims, France
dc.creator.affilliationUniversite de Reims. U. F. R. de Pharmacie. Laboratoire de Paleoparasitologie. Reims, France.
dc.creator.affilliationUniversite de Reims. U. F. R. de Pharmacie. Laboratoire de Paleoparasitologie. Reims, France.
dc.creator.affilliationFundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sergio Arouca. Rio de Janeiro, RJ, Brasil.
dc.creator.affilliationFundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sergio Arouca. Rio de Janeiro, RJ, Brasil.
dc.subject.enPaleoparasitology
dc.subject.enCoprolites
dc.subject.enAncient Faeces
dc.subject.enAmebiasis
dc.subject.enELISA
dc.subject.decsAmebiasis
dc.subject.decsEnzyme-Linked Immunosorbent Assay
dc.identifier.eissn1878-3503
Appears in Collections:ENSP - Artigos de Periódicos

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