Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/31687
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dc.contributor.authorBatista, Cassiano Martin
dc.contributor.authorKessler, Rafael Luis
dc.contributor.authorEger, Iriane
dc.contributor.authorSoares, Maurilio José
dc.date.accessioned2019-02-15T16:53:05Z
dc.date.available2019-02-15T16:53:05Z
dc.date.issued2018
dc.identifier.citationBATISTA, Cassiano Martin et al. Treatment of Trypanosoma cruzi with 2-bromopalmitate alters morphology, endocytosis, differentiation and infectivity. BMC Cell Biology, v. 19, n. 19, p. 1-16, 2018.
dc.identifier.issn1471-2121
dc.identifier.urihttps://www.arca.fiocruz.br/handle/icict/31687
dc.language.isopor
dc.publisherBMC
dc.rightsopen access
dc.subject.otherTrypanosoma cruzi
dc.titleTreatment of Trypanosoma cruzi with 2-bromopalmitate alters morphology, endocytosis, differentiation and infectivity
dc.typeArticle
dc.identifier.doi10.1186/s12860-018-0170-3
dc.description.abstractenThe palmitate analogue 2-bromopalmitate (2-BP) is a non-selective membrane tethered cysteine alkylator of many membrane-associated enzymes that in the last years emerged as a general inhibitor of protein S-palmitoylation. Palmitoylation is a post-translational protein modification that adds palmitic acid to a cysteine residue through a thioester linkage, promoting membrane localization, protein stability, regulation of enzymatic activity, and the epigenetic regulation of gene expression. Little is known on such important process in the pathogenic protozoan Trypanosoma cruzi, the etiological agent of Chagas disease. The effect of 2-BP was analyzed on different developmental forms of Trypanosoma cruzi. The IC50/48 h value for culture epimastigotes was estimated as 130 μM. The IC50/24 h value for metacyclic trypomastigotes was 216 nM, while for intracellular amastigotes it was 242 μM and for cell derived trypomasigotes was 262 μM (IC50/24 h). Our data showed that 2-BP altered T. cruzi: 1) morphology, as assessed by bright field, scanning and transmission electron microscopy; 2) mitochondrial membrane potential, as shown by flow cytometry after incubation with rhodamine-123; 3) endocytosis, as seen after incubation with transferrin or albumin and analysis by flow cytometry/fluorescence microscopy; 4) in vitro metacyclogenesis; and 5) infectivity, as shown by host cell infection assays. On the other hand, lipid stress by incubation with palmitate did not alter epimastigote growth, metacyclic trypomastigotes viability or trypomastigote infectivity. Our results indicate that 2-BP inhibits key cellular processes of T. cruzi that may be regulated by palmitoylation of vital proteins and suggest a metacyclic trypomastigote unique target dependency during the parasite development.
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Celular. Curitiba, PR, Brasil.
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Genômica Funcional. Curitiba, PR, Brasil. / Instituto de Biologia Molecular do Paraná. Laboratório de Biotecnologia Celular de Mamíferos. Curitiba, PR, Brasil.
dc.creator.affilliationUniversidade Estadual de Ponta Grossa. Departamento de Biologia Geral. Ponta Grossa, PR, Brasil.
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Celular. Curitiba, PR, Brasil.
dc.subject.enCell Differentiation
dc.subject.enEndocytosis
dc.subject.enLipoylation
dc.subject.en2-Bromopalmitate
dc.subject.enChagas Disease
dc.subject.esDiferenciación Celular
dc.subject.esEndocitosis
dc.subject.esLipoilación
dc.subject.decsDiferenciação Celular
dc.subject.decsEndocitose
dc.subject.decsLipoilação
dc.subject.decsDoença de Chagas
Appears in Collections:PR - ICC - Artigos de Periódicos

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