Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/31695
Title: Electrochemical immunosensor for differential diagnostic of Wuchereria bancrofti using a synthetic peptide
Authors: Prado, Isis C.
Mendes, Verônica G.
Souza, André L. A.
Dutra, Rosa F.
De Simone, Salvatore Giovanni
Affilliation: Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Instituto Nacional de Ciência e Tecnologia para Inovação em Doenças Negligenciadas. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica Experimental e Computacional de Fármacos. Rio de Janeiro, RJ, Brasil.
Universidade Federal de Pernambuco. Laboratório de Engenharia Biomédica. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Instituto Nacional de Ciência e Tecnologia para Inovação em Doenças Negligenciadas. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica Experimental e Computacional de Fármacos. Rio de Janeiro, RJ, Brasil / Universidade Federal Fluminense. Instituto de Biologia. Departamento de Biologia Celular e Molecular. Niterói, RJ, Brasil.
Abstract: Lymphatic filariasis (LF) is a neglected tropical disease transmitted by mosquitoes and the second cause of permanent disability leading to a significant morbidity and mortality rate. Previously, we have identified epitopes of the filarial abundant larval transcript-2 (ALT-2) protein using a microarray mapping. In this study, one of the epitopes (Wb/ALT2-A5) was used to construct an electrochemical immunosensor. Electrochemical technique of cyclic voltammetry was performed for detecting the signal generated by the interaction between the (Wb/ALT2-A5) peptide and circulating antibodies of serum human samples. (Wb/ALT2-A5) epitope antigens were successfully immobilized on the working electrode of a screen-printed carbon electrode (SPCE) by their amine groups via chitosan film by coupling with glutaraldehyde as crosslinker. After the sensor ready, a pool of human sera infected with Wuchereria bancrofti was added to its surface. Electrochemical responses were generated by applying a potential of - 0.6 to 0.6 V, scan rate of 0.025 V/s. A detection limit of 5.0 µg mL-1 for the synthetic peptides (Wb/ALT2-A5) and 0.002 µg mL-1 for human serum, with a sensitivity of 1.86 µA. The performance of this assay was successfully tested in human serum samples from infected and healthy patients. Thus, this proposed immunosensor, which is able to identify circulating antibodies, can be applied to the diagnosis of the W. bancrofti parasitic disease.
Keywords: Electroanalytical immunoassay
Electroanalytic
Wuchereria bancrofti
Diagnostic
Epitope
keywords: Wuchereria bancrofti
Epitopo
Imunoensaio eletroanalítico
Eletroanálise
Diagnóstico
Issue Date: 2018
Publisher: Elsevier
Citation: PRADO, Isis C. et al. Electrochemical immunosensor for differential diagnostic of Wuchereria bancrofti using a synthetic peptide. Biosensors and Bioelectronics, v. 113, p. 9-15, Apr. 2018.
DOI: 10.1016/j.bios.2018.04.045
ISSN: 0956-5663
Copyright: restricted access
Appears in Collections:INI - Artigos de Periódicos
CDTS - Artigos de Periódicos
IOC - Artigos de Periódicos

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