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APPLICATION OF SENSITIVE AND SPECIFIC MOLECULAR METHODS TO UNCOVER GLOBAL DISSEMINATION OF THE MAJOR RDRIO SUBLINEAGE OF THE LATIN AMERICAN-MEDITERRANEAN MYCOBACTERIUM TUBERCULOSIS SPOLIGOTYPE FAMILY
Gibson, Andrea L. et al. | Date Issued: 2008
Author
Gibson, Andrea L.
Huard, Richard C.
van Pittius, Nicolaas C.. Gey
Lazzarini, Luiz Claudio Oliveira
Driscoll, Jeffrey
Kurepina, Natalia
Zozio, Thierry
Sola, Christophe
Spindola, Silvana Miranda
Kritski, Afrânio L.
Fitzgerald, Daniel
Kremer, Kristin
Mardassi, Helmi
Chitale, Poonam
Brinkworth, Jessica
Viedma, Dario Garcia de
Gicquel, Brigitte
Pape, Jean W.
van Soolingen, Dick
Kreiswirth, Barry N.
Warren, Robin M.
van Helden, Paul D.
Rastogi, Nalin
Suffys, Philip N.
Silva, Jose Lapa e
Ho, John L..
Affilliation
Weill Medical College of Cornell University. Department of Medicine. Division of International Medicine and Infectious Disease. New York, NY, USA.
Weill Medical College of Cornell University. Department of Medicine. Division of International Medicine and Infectious Disease. New York, NY, USA / Columbia University Medical Center. New York-Presbyterian Hospital. Clinical Microbiology Service and the Department of Pathology. New York, NY, USA.
Stellenbosch University. Faculty of Health Sciences. Department of Biomedical Sciences. Division of Molecular Biology and Human Genetics. US/MRC Centre for Molecular and Cellular Biology. DST/NRF Centre of Excellence in Biomedical Tuberculosis Research. Cape Town, South Africa.
Weill Medical College of Cornell University. Department of Medicine. Division of International Medicine and Infectious Disease. New York, NY, USA / Universidade Federal do Rio de Janeiro. Instituto de Doenças Toráxicas. Rio de Janeiro, RJ, Brasil.
New York State Department of Health. Wadsworth Center. Albany, New York, USA.
Public Health Research Institute. Newark, New Jersey, USA.
Institut Pasteur de Guadeloupe. Unité de la Tuberculose et des Mycobactéries. Guadeloupe.
Institut Pasteur de Guadeloupe. Unité de la Tuberculose et des Mycobactéries. Guadeloupe / Institut Pasteur. Unité de Génétique Mycobactérienne. Paris, France.
Universidade Federal de Minas Gerais. Faculdade de Medicina. Departamento de Clínica Médica/Pneumologia. Belo Horizonte, MG, Brasil.
Universidade Federal do Rio de Janeiro. Instituto de Doenças Toráxicas. Rio de Janeiro, RJ, Brasil.
Weill Medical College of Cornell University. Department of Medicine. Division of International Medicine and Infectious Disease. New York, NY, USA / GHESKIO Centers. Port-au-Prince, Haiti.
National Institute of Public Health and the Environment. Bilthoven, The Netherlands.
Institut Pasteur de Tunis. Laboratoire des Mycobactéries. Tunisia.
Weill Medical College of Cornell University. Department of Medicine. Division of International Medicine and Infectious Disease. New York, NY, USA.
Weill Medical College of Cornell University. Department of Medicine. Division of International Medicine and Infectious Disease. New York, NY, USA / City University of New York/New York Consortium in Evolutionary Primatology. Department of Anthropology, Graduate School and University Center, New York, New York.
Hospital Gregorio Maranón. Servicio de Microbiología Clínica y Enfermedades Infecciosas. Madrid, Spain / CIBER Enfermedades Respiratorias. Madrid, Spain.
Institut Pasteur. Unité de Génétique Mycobactérienne. Paris, France.
GHESKIO Centers. Port-au-Prince, Haiti.
National Institute of Public Health and the Environment. Bilthoven, The Netherlands.
Public Health Research Institute. Newark, New Jersey, USA.
Stellenbosch University. Faculty of Health Sciences. Department of Biomedical Sciences. Division of Molecular Biology and Human Genetics. US/MRC Centre for Molecular and Cellular Biology. DST/NRF Centre of Excellence in Biomedical Tuberculosis Research. Cape Town, South Africa.
Stellenbosch University. Faculty of Health Sciences. Department of Biomedical Sciences. Division of Molecular Biology and Human Genetics. US/MRC Centre for Molecular and Cellular Biology. DST/NRF Centre of Excellence in Biomedical Tuberculosis Research. Cape Town, South Africa.
Institut Pasteur de Guadeloupe. Unité de la Tuberculose et des Mycobactéries. Guadaloupe.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular Aplicada em Micobactérias. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Instituto de Doenças Toráxicas. Rio de Janeiro, RJ, Brasil.
Weill Medical College of Cornell University. Department of Medicine. Division of International Medicine and Infectious Disease. New York, NY, USA.
Abstract
The Latin American-Mediterranean (LAM) family of Mycobacterium tuberculosis is believed to be the cause of approximately 15% of tuberculosis cases worldwide. Previously, we defined a prevalent sublineage of the LAM family in Brazil by a single characteristic genomic deletion designated RD(Rio). Using the Brazilian strains, we pinpoint an Ag85C(103) single nucleotide polymorphism (SNP) (screened by restriction fragment length polymorphism [RFLP] analysis) that correctly identified all LAM family strains. Importantly, all RD(Rio) strains concomitantly possessed the RD174 deletion. These genetic signatures, along with a newly developed multiplex PCR for rapid differentiation between "wild-type" and RD(Rio) strains, were then used to analyze an international collection of M. tuberculosis strains. RD(Rio) M. tuberculosis was identified from four continents involving 11 countries. Phylogenetic analysis of the IS6110-RFLP patterns from representative RD(Rio) and LAM strains from Brazil, along with all representative clusters from a South African database, confirmed their genetic relatedness and transcontinental transmission. The Ag85C(103) SNP RFLP, as compared to results obtained using a PCR method targeting a LAM-restricted IS6110 element, correctly identified 99.8% of LAM spoligotype strains. Together, these tests were more accurate than spoligotyping at categorizing strains with indefinable spoligotypes and segregated true LAM strains from those with convergent spoligotypes. The fact that RD(Rio) strains were identified worldwide highlights the importance of this LAM family sublineage and suggests that this strain is a global threat that should be specifically targeted by public health resources. Our provision of simple and robust molecular methods will assist the evaluation of the LAM family and the RD(Rio) sublineage.
Keywords in Portuguese
Mycobacterium tuberculosis
RDRio
Métodos Moleculares Sensíveis e específicos
Divulgação Global
Aplicação
 
Keywords
Mycobacterium tuberculosis
RDRio
Latin American-Mediterranean (LAM) spoligotype family
Global Dissemination
Specific Molecular Methods
 
Publisher
American Society for Microbiology
Citation
GIBSON, Andrea L. et al. Application of Sensitive and Specific Molecular Methods To Uncover Global Dissemination of the Major RDRio Sublineage of the Latin American-Mediterranean Mycobacterium tuberculosis Spoligotype Family. Journal of Clinical Microbiology, v. 46, n. 4, p. 1259-1267, Apr. 2008.
DOI
10.1128/JCM.02231-07
ISSN
0095-1137