Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/32482
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dc.contributor.authorRodrigues, Elizabeth C. P.
dc.contributor.authorSouza, Mauro C. L.
dc.contributor.authorToledo, Sandro S.
dc.contributor.authorBarbosa, Celso G.
dc.contributor.authorReis, Eliane M. F.
dc.contributor.authorRodrigues, Dalia P.
dc.contributor.authorLázaro, Norma S.
dc.date.accessioned2019-04-12T19:36:44Z
dc.date.available2019-04-12T19:36:44Z
dc.date.issued2011
dc.identifier.citationRODRIGUES, Elizabeth C. P. et al. Effects of gamma irradiation on the viability and phenotypic characteristics of Salmonella Enteritidis inoculated into specific-pathogen-free eggs. Journal of Food Protection, Ames, v. 74, n. 12, p. 2031-2038, 2011.
dc.identifier.issn1944-9097
dc.identifier.urihttps://www.arca.fiocruz.br/handle/icict/32482
dc.language.isoeng
dc.rightsrestricted access
dc.titleEffects of gamma irradiation on the viability and phenotypic characteristics of Salmonella Enteritidis inoculated into specific-pathogen-free eggs
dc.typeArticle
dc.identifier.doi10.4315/0362-028X.JFP-11-086
dc.description.abstractenThe goal of this study was to determine the effects of various levels of gamma irradiation on the phenotypic characteristics of 20 strains of Salmonella Enteritidis inoculated separately into specific-pathogen-free shell eggs. Bacterial strains were inoculated into egg yolks and exposed to (60)Co radiation at doses of 0.49 to 5.0 kGy. The eggs were maintained at 25°C and analyzed for the presence of Salmonella on days 1, 2, 4, and 7, and the recovered Salmonella isolates were characterized biochemically. All strains were resistant to doses of 0.49, 0.54, 0.59, 0.8, and 1 kGy; colony counts were ≥10(5) CFU/ml of egg yolk except for one strain, which was detected at 96 h and at 7 days after irradiation at 1 kGy, with a population reduction of 2 log CFU/ml. For the other evaluated doses, 12 strains (60.0%) were resistant at 1.5 kGy and 7 strains (35.0%) were resistant at 3.0 kGy. Among all analyzed strains, 5.0 kGy was more effective for reducing and/or eliminating the inoculated bacteria; only two (10%) strains were resistant to this level of irradiation. Salmonella colony counts were significantly reduced (P < 0.01) with increasing doses from the day 1 to 7 of observation, when microbial growth peaked. Loss of mobility, lactose fermentation, citrate utilization, and hydrogen sulfide production occurred in some strains after irradiation independent of dose and postirradiation storage time. Increases in antibiotic susceptibility also occurred: seven strains became sensitive to β-lactams, two strains became sensitive to antifolates, and one strain each became sensitive to fluoroquinolone, phenicol, nitrofurans, tetracyclines, and aminoglycosides. The results indicate that up to 5.0 kGy of radiation applied to shell eggs inoculated with Salmonella Enteritidis at 4 log CFU per egg is not sufficient for complete elimination of this pathogen from this food matrix.
dc.creator.affilliationFundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Enterobactérias. Rio de Janeiro, RJ, Brasil / Universidade Federal Rural do Rio de Janeiro. Rio de Janeiro, Brasil.
dc.creator.affilliationUniversidade do Rio de Janeiro. Laboratório de Instrumentação Nuclear. Centro de Tecnologia. Rio de Janeiro, Brasil.
dc.creator.affilliationUniversidade do Rio de Janeiro. Laboratório de Instrumentação Nuclear. Centro de Tecnologia. Rio de Janeiro, Brasil.
dc.creator.affilliationUniversidade Federal Rural do Rio de Janeiro. Rio de Janeiro, Brasil.
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Enterobactérias. Rio de Janeiro, RJ, Brasil.
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Enterobactérias. Rio de Janeiro, RJ, Brasil.
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Enterobactérias. Rio de Janeiro, RJ, Brasil.
dc.subject.enGamma Irradiation
dc.subject.enEffects
dc.subject.enPhenotypic Characteristics
dc.subject.enSalmonella Enteritidis Inoculated
dc.subject.enSpecific-Pathogen-Free Eggs
Appears in Collections:IOC - Artigos de Periódicos
Biomanguinhos - Artigos de Periódicos

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