Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/32489
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dc.contributor.authorRoma, Eric Henrique
dc.contributor.authorMacedo, Juan Pereira
dc.contributor.authorGoes, Grazielle Ribeiro
dc.contributor.authorGonçalves, Juliana Lauar
dc.contributor.authorCastro, Waldionê de
dc.contributor.authorCisalpino, Daniel
dc.contributor.authorVieira, Leda Quercia
dc.date.accessioned2019-04-15T14:34:06Z
dc.date.available2019-04-15T14:34:06Z
dc.date.issued2016
dc.identifier.citationROMA, Eric Henrique et al. Impact of reactive oxygen species (ROS) on the control of parasite loads and inflammation in Leishmania amazonensis infection. Parasites and Vectors, v. 9, p. 1-13, 2016.
dc.identifier.urihttps://www.arca.fiocruz.br/handle/icict/32489
dc.language.isoeng
dc.publisherBMC
dc.rightsopen access
dc.titleImpact of reactive oxygen species (ROS) on the control of parasite loads and inflammation in Leishmania amazonensis infection
dc.typeArticle
dc.identifier.doi10.1186/s13071-016-1472-y
dc.description.abstractenBackground: Reactive oxygen species (ROS) protect the host against a large number of pathogenic microorganisms. ROS have different effects on parasites of the genus Leishmania: some parasites are susceptible to their action, while others seem to be resistant. The role of ROS in L. amazonensis infection in vivo has not been addressed to date. Methods: In this study, C57BL/6 wild-type mice (WT) and mice genetically deficient in ROS production by phagocytes (gp91phox−/−) were infected with metacyclic promastigotes of L. amazonensis to address the effect of ROS in parasite control. Inflammatory cytokines, parasite loads and myeloperoxidase (MPO) activity were evaluated. In parallel, in vitro infection of peritoneal macrophages was assessed to determine parasite killing, cytokine, NO and ROS production. Results: In vitro results show induction of ROS production by infected peritoneal macrophages, but no effect in parasite killing. Also, ROS do not seem to be important to parasite killing in vivo, but they control lesion sizes at early stages of infection. IFN-γ, TNF-α and IL-10 production did not differ among mouse strains. Myeloperoxidase assay showed augmented neutrophils influx 6 h and 72 h post - infection in gp91phox−/− mice, indicating a larger inflammatory response in gp91phox−/− even at early time points. At later time points, neutrophil numbers in lesions correlated with lesion size: larger lesions in gp91phox−/− at earlier times of infection corresponded to larger neutrophil infiltrates, while larger lesions in WT mice at the later points of infection also displayed larger numbers of neutrophils. Conclusion: ROS do not seem to be important in L. amazonensis killing, but they regulate the inflammatory response probably by controlling neutrophils numbers in lesions.
dc.creator.affilliationUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil / Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil.
dc.creator.affilliationUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil
dc.creator.affilliationUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil
dc.creator.affilliationUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil / Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil.
dc.creator.affilliationUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil / National Institutes of Health, NIAID. Laboratory of Malaria and Vector Research. Vector Molecular Biology Section. Rockville, MD, USA.
dc.creator.affilliationUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brasil.
dc.creator.affilliationUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil.
dc.subject.enLeishmania amazonensis
dc.subject.enROS
dc.subject.enNOX2
dc.subject.enNeutrophils
dc.subject.enInflammation
dc.identifier.eissn1756-3305
Appears in Collections:INI - Artigos de Periódicos

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