Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/33007
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dc.contributor.authorPastor, André Filipe
dc.contributor.authorRocha, Abraham
dc.contributor.authorCassemiro, Klécia de Melo
dc.contributor.authorTenório, Marli
dc.contributor.authorMelo, Paula
dc.contributor.authorGrilis, Maria Rosângela
dc.contributor.authorRhuama, Maressa
dc.contributor.authorRezende, Antonio Mauro
dc.contributor.authorMelo Neto, Osvaldo Pompilio de
dc.contributor.authorMarques, Ernesto
dc.contributor.authorDhalia, Rafael
dc.date.accessioned2019-05-09T12:49:38Z
dc.date.available2019-05-09T12:49:38Z
dc.date.issued2018
dc.identifier.citationPASTOR, André Filipe et al. Evaluation of the Recombinant Antigens Wb14 and WbT for the Capture Antibody Diagnosis of Lymphatic Filariasis. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 113, n. 5, p. 1-8, mar. 2018.
dc.identifier.issn1678-8060
dc.identifier.urihttps://www.arca.fiocruz.br/handle/icict/33007
dc.language.isoeng
dc.rightsopen access
dc.titleEvaluation of the recombinant antigens Wb14 and WbT for the capture antibody diagnosis of lymphatic filariasis
dc.typeArticle
dc.identifier.doi10.1590/0074-02760170435
dc.description.abstractenBACKGROUND Lymphatic filariasis (LF) is a parasitic disease caused mainly by the Wuchereria bancrofti worm and that affects up to 120 million people worldwide. LF is the second cause of chronic global deformity, responsible for 15 million people with lymphedema (elephantiasis) and 25 million men with scrotal hydrocele. Its diagnosis is still associated with numerous difficulties, such as the sample collection periods (microfilaria nocturnal periodicity) and limited diagnostic kits. OBJECTIVES The aim of this work was to evaluate two recombinant antigens (Wb14 and WbT) as part of an enzyme-linked immunosorbent assay (ELISA) based antibody capture tests for LF. METHODS The recombinant antigens rWb14 and rWbT were expressed in Escherichia coli BL21 and an antibody capture ELISA was performed. For this, sera were used from microfilaremic individuals with W. bancrofti (MF), chronic pathology (CP), individuals infected with Strongyloides (SP) and healthy controls from endemic (EN) and non-endemic (NE) areas. FINDINGS Both tests showed similar results, with 90% sensitivity and 96.6% specificity. In comparison with the BM14 ELISA commercial test, the Wb14 and WbT antigens performed with identical sensitivity but greater specificity. Reduced positivity with the CP suggested a potential to monitor cure. This was not confirmed, however, when sera from individuals up to seven years after treatment were assayed. MAIN CONCLUSIONS The Wb14 and WbT ELISAs were considered efficient and promising diagnostic tests. Due to the importance of antibody capture analysis to evaluate the Global Program to Eliminate Lymphatic Filariasis (GPELF), the tests proposed here appear as great alternatives to the available commercial system.
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil / Universidade Federal de Pernambuco. Recife, PE, Brasil / Instituto Federal de Ciência e Tecnologia do Sertão Pernambucano. Campus Floresta, PE, Brasil.
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil / Fundação Oswaldo Cruz. Instituto Aggeu Magalhães, Serviço de Referência Nacional em Filarioses. Recife, PE, Brasil / Hospital Otávio de Freitas. Recife, PE, Brasil.
dc.creator.affilliationInstituto Nacional de Câncer. Laboratório de Patologia Clínica. Rio de Janeiro, RJ, Brasil
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil / Fundação Oswaldo Cruz. Instituto Aggeu Magalhães, Serviço de Referência Nacional em Filarioses. Recife, PE, Brasil.
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil / Fundação Oswaldo Cruz. Instituto Aggeu Magalhães, Serviço de Referência Nacional em Filarioses. Recife, PE, Brasil.
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil / Universidade Federal de Pernambuco. Recife, PE, Brasil.
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil / Universidade Federal de Pernambuco. Recife, PE, Brasil.
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.
dc.creator.affilliationFundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil / Universidade Federal de Pernambuco. Recife, PE, Brasil.
dc.subject.enImmunodiagnostic
dc.subject.enWuchereria bancrofti
dc.subject.enELISA
dc.subject.enBiotechnology
dc.subject.decsAnimais
dc.subject.decsAnticorpos, Helmintos / sangue
dc.subject.decsAntígenos, Helmintos / sangue
dc.subject.decsAntígenos de Helmintos / imunologia
dc.subject.decsEstudos de caso-controle
dc.subject.decsElefantíase, filarial / diagnóstico
dc.subject.decsHumanos
dc.subject.decsCurva ROC
dc.subject.decsProteínas Recombinantes / sangue
dc.subject.decsProteínas Recombinantes / imunologia
dc.subject.decsReprodutibilidade dos resultados
dc.subject.decsSensibilidade e Especificidade
dc.subject.decsWuchereria bancrofti / imunologia
Appears in Collections:PE - IAM - Artigos de Periódicos




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