Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/33009
Title: HLA-G is expressed in intestinal samples of ulcerative colitis and Crohn's disease patients and HLA-G5 expression is differentially correlated with TNF and IL-10 cytokine expression
Authors: Gomes, Renan Garcia
Brito, Carlos Alexandre Antunes de
Martinelli, Valéria Ferreira
Santos, Rossana Nascimento dos
Gomes, Fabiana Oliveira dos Santos
Peixoto, Christina Alves
Crispim, Janaína Oliveira
Diniz, George Tadeu Nunes
Donadi, Eduardo Antônio
Lucena-Silva, Norma
Affilliation: Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.
Federal University of Pernambuco. School of Medicine. Department of Internal Medicine. Recife, PE, Brazil.
Federal University of Pernambuco. School of Medicine. Department of Internal Medicine. Recife, PE, Brazil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.
Federal University of Rio Grande do Norte. Department of Pharmacology. Natal, RN, Brazil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.
University of São Paulo. Medical School of Ribeirão Preto. Department of Internal Medicine. Ribeirão Preto, SP, Brazil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.
Abstract: HLA-G is an immunomodulatory molecule that can be produced by epithelial cells. Considering that TNF and IL-10 participate in bowel inflammatory disorders and that both cytokines modulate HLA-G, we evaluated HLA-G, TNF and IL-10 mRNA expression by qPCR and HLA-G protein levels by immunohistochemistry in two intestinal samples exhibiting different degree of inflammation within a patient suffering from Crohn's disease (CD) or ulcerative colitis (UC). Tissue HLA-G5 (P < 0.0001), TNF (P = 0.0004) and IL-10 (P = 0.0169) mRNA expression levels were higher in intestinal areas exhibiting intense inflammation compared to areas of low inflammation, and HLA-G protein levels were not associated with degree of mucosal inflammation. In CD, the expression of TNF was correlated with IL-10 in low inflamed areas, exhibiting a TNF:IL-10 ratio = 3, but in inflamed areas the ratio increased to 9-folds. In UC, the expression of TNF was correlated to IL-10, irrespective of the inflammation grade, with little variation of the TNF:IL-10 ratio in the various inflamed areas. TNF and IL-10 expression was correlated with HLA-G5 expression in mild inflamed areas. Both CD and UC samples exhibited gene and protein expression of HLA-G; and the HLA-G5 expression is differentially correlated with TNF and IL-10 levels depending on the type of the underlying inflammatory bowel disorder.
Keywords: Crohn’s disease
HLA-G
IL-10
Inflammatory bowel disease
TNF
Ulcerative colitis
DeCS: Adolescente
Adulto
Envelhecido
Colite Ulcerativa / imunologia
Doença de Crohn / imunologia
Progressão da doença
Células Epiteliais / fisiologia
Fêmea
Antígenos HLA-G / metabolismo
Humanos
Imunohistoquímica
Imunomodulação
Interleucina-10 / genética
Interleucina-10 / metabolismo
Mucosa Intestinal / metabolismo
Intestinos / patologia
Masculino
Meia idade
Reação em Cadeia da Polimerase
RNA, mensageiro / análise
Fator alfa de necrose tumoral / genética
Fator de Necrose Tumoral alfa / metabolismo
Adulto jovem
Issue Date: 2018
Citation: GOMES, R. G. et al. HLA-G Is Expressed in Intestinal Samples of Ulcerative Colitis and Crohn’s Disease Patients and HLA-G5 Expression Is Differentially Correlated with TNF and IL-10 Cytokine Expression. Human Immunology, v. 79, n. 6, p. 477–484, June 2018.
DOI: 10.1016/j.humimm.2018.03.006
ISSN: 1879-1166
Copyright: restricted access
Appears in Collections:PE - IAM - Artigos de Periódicos

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