| Author | Sampaio, M. C. R. | |
| Author | Barbosa, A. F. | |
| Author | Este, M. G. | |
| Author | Pirmez, C. | |
| Author | Bello, A .R. | |
| Author | Traub-Csekö, Y. M. | |
| Access date | 2019-05-16T11:02:53Z | |
| Available date | 2019-05-16T11:02:53Z | |
| Document date | 2009 | |
| Citation | SAMPAIO, M. C. R. et al. A 245 kb mini-chromosome impacts on Leishmania braziliensis infection and survival. Biochemical and Biophysical Research Communications, v. 382, p. 74–78, 2009. | pt_BR |
| ISSN | 0006-291X | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/33099 | |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Pteridinas | pt_BR |
| Subject in Portuguese | Amplificação genômica | pt_BR |
| Subject in Portuguese | Amplificação LD1 | pt_BR |
| Subject in Portuguese | Cromossomo pequeno | pt_BR |
| Subject in Portuguese | Transportador de biopterina | pt_BR |
| Title | A 245kb mini-chromosome impacts on Leishmania braziliensis infection and survival | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1016/j.bbrc.2009.02.128 | |
| Abstract | Leishmania (V.) braziliensis, the causative agent of mucocutaneous leishmaniasis in the New World, may present an LD1 type genomic amplification that appears as a small 245 kb linear chromosome, and is not clearly associated to the presence of a selection agent. A bt1 gene, codifying for a biopterin transporter protein, was identified in this small chromosome. Leishmania are auxotrophic for pterins and one of the proposed explanations for the appearance of this amplification is the improvement of biopterin capture by the parasite. We analyzed some biological aspects of two lineages of L. braziliensis strain M2903, with and without the small amplified chromosome. We showed differences in infectivity of these lineages, in macrophages and the insect vector Lutzomyia longipalpis, as well as in the uptake and metabolization of intermediates of the Leishmania biopterin salvage pathway. Our results suggest that the genomic amplification favors survival due to improved biopterin capture and at the same time hinders the infective capability, suggesting that within a population different parasites can perform different roles. | pt_BR |
| Affilliation | Universidade do Estado do Rio de Janeiro. Instituto de Biologia Roberto Alcântara Gomes. Departamento de Biologia Celular. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Parasitas e Vetores. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Transmissores de Leishmanioses. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunopatologia. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunopatologia. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Universidade do Estado do Rio de Janeiro. Faculdade de Ciências Médicas. Departamento de Microbiologia, Imunologia e Parasitologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Parasitas e Vetores. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Genomic amplification | pt_BR |
| Subject | Small chromosome | pt_BR |
| Subject | Biopterin transporter | pt_BR |
| Subject | Pteridines | pt_BR |
| Subject | LD1 amplification | pt_BR |
| DeCS | Pteridinas | pt_BR |
| e-ISSN | 1090-2104 | |
| Embargo date | 2022-01-01 | |
