Objective: To describe the use of intralesional amphotericin B inlocalised lesions for the treatment of 26 cats from Rio de Janeiro, Brazil, with sporotrichosis refractory to oral itraconazole. Design: Uncontrolled intervention study. Method: The 26 cats in this study were diagnosed with sporotri-chosis, confirmed by isolation of Sporothrix schenckii, and presented residual localised skin lesions refractory to treatment with oral itraconazole for a minimum period of 8 weeks. The animals received weekly applications of intralesional amphotericin B in conjunction with oral itraconazole. In cases of owner unavailability, a maximumof 2 weeks between the infiltrations was accepted. Results: Twenty-two (84.6%) of the 26 treated cats achieved clini-cal remission, 16 (72.7%) of which were cured, and in the remainingsix (27.3%) the lesions recurred at the same site. Lack of clinical response was observed in one animal and three owners abandonedtreatment. Conclusion: The proposed therapeutic regimen is an adjunctive treatment option for cats with sporotrichosis presenting as residualskin lesions refractory to itraconazole.