Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/33608
Title: Genetic characterization of environmental isolates of the Cryptococcus neoformans species complex from Brazil
Authors: Trilles, L.
Lazéra, M.
Wanke, B.
Theelen, B.
Boekhout, T.
Affilliation: Fundação Oswaldo Cruz. lnstituto de Pesquisa Clinica Evandro Chagas. Serviqo de Micologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. lnstituto de Pesquisa Clinica Evandro Chagas. Serviqo de Micologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. lnstituto de Pesquisa Clinica Evandro Chagas. Serviqo de Micologia. Rio de Janeiro, RJ, Brasil.
Centraalbureau voor Schimmelcultures. Utrecht, The Netherlands.
Centraalbureau voor Schimmelcultures. Utrecht, The Netherlands.
Abstract: The genetic affiliation of a large number of isolates of the Cryptococcus neofornqans species complex from environmental sources in Brazil has been investigated using amplified fragment length polymorphism (AFLP). The strains of C. neoformans isolated from a single tree, as well as from neighbouring trees, showed high similarity values ( > 95%) of their AFLP patterns, thus suggesting considerable genetic homogeneity. The majority of isolates of C. neoforrnans belonged to AFLP genotype 1, and had serotype A and mating type a ( = C. neoformans var. grubii). Three isolates belonged to AFLP genotype 2, with serotype D and mating type a ( = C. neofornzans var. neoformans). One isolate, obtained from a building in Rio de Janeiro inhabited by pigeons, belonged to the AD hybrid AFLP genotype 3. All isolates from trees of C. neoforrnans var. gattii ( = C. gattii) belonged to AFLP genotype 6, and their banding patterns showed relatively low genetic homogeneity with a similarity value of about 76%. Isolates of this genotype occupy an environmental niche in the Americas, and they may cause disease in non-AIDS and AIDS patients as well.
keywords: Cryptococcus gattii
Cryptococcus neoformans
Ecology
Genotyping
Trees
Yeast
Issue Date: 2003
Publisher: Oxford University Press
Citation: TRILLES, L. et al. Genetic characterization of environmental isolates of the Cryptococcus neoformans species complex from Brazil.Medical Mycology, v. 41, p. 383-390, 2003.
DOI: 10.1080/1369378031000137206
ISSN: 1369-3786
Copyright: restricted access
Appears in Collections:INI - Artigos de Periódicos

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