Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/34686
Title: Leishmania infantum exo-antigens: application toward serological diagnosis of visceral leishmaniasis
Authors: Saliba, Juliana Wilke
Lopes, Karine Ferreira
Pereira, Rosiane Aparecida Silva
Teixeira, Luciana A. S.
Oliveira, Edward José de
Affilliation: Fundação Oswaldo Cruz. Instituto René Rachou. Pesquisas Clínicas e Políticas Públicas em Doenças Infecciosas e Parasitárias. Belo Horizonte, MG, Brasil.
Fundação Oswaldo Cruz. Instituto René Rachou. Pesquisas Clínicas e Políticas Públicas em Doenças Infecciosas e Parasitárias. Belo Horizonte, MG, Brasil.
Fundação Oswaldo Cruz. Instituto René Rachou. Biologia e Imunologia de Doenças Infecciosas e Parasitárias. Belo Horizonte, MG, Brasil.
Universidade Federal do Triângulo Mineiro. Faculdade de Medicina. Uberaba, MG, Brasil.
Fundação Oswaldo Cruz. Instituto René Rachou. Pesquisa Clínica e Políticas Públicas em Doenças Infecciosas e Parasitárias. Belo Horizonte, MG, Brasil.
Abstract: The laboratory diagnosis of visceral leishmaniasis (VL) presents limitations related to its sensibility and/or specificity. In this context, the aim of this study was to evaluate an enzyme-linked immunoassay to detect IgG antibodies against Leishmania infantum exo-antigens for diagnosis of VL, called ELISA-Exo. This assay was applied in 309 masked serum samples from VL, tegumentary leishmaniasis, Chagas disease, schistosomiasis mansoni, malaria patients, and healthy individuals. The results were compared with those from ELISA using rK39 as antigen (ELISA-rK39). The ELISA assays presented sensitivity of 96.8% and 98.4% (p = 0.68), specificity of 92.4% for both, and diagnostic accuracy of 94.2% and 94.8% (p = 0.48) by the ELISA-Exo and ELISA-rK39, respectively. An excellent agreement beyond chance (Kappa index = 0.82) was obtained when the results from ELISA assays were cross-tabulated. The Western blotting showed that false-positive results presented by ELISA-Exo probably were produced by cross-reactivity of antigens shared with the species of the family Trypanosomatidae. In the future, an immunoproteomic approach can contribute for identification of main immunoreactive L. infantum exo-antigens.
Keywords: ELISA-Exo
Immunoreactive exo-antigens
Serological tests
Visceral leishmaniasis
Issue Date: 2019
Publisher: Springer International
Citation: SALIBA, Juliana Wilke et al. Leishmania infantum exo-antigens: application toward serological diagnosis of visceral leishmaniasis. Parasitology Research, v. 118, n. 7, p. 2317-2323, 2019.
DOI: 10.1007/s00436-019-06352-4
ISSN: 0932-0113
Copyright: restricted access
Appears in Collections:MG - IRR - Artigos de Periódicos

Files in This Item:
File Description SizeFormat 
Leishmania infantum exo-antigenspdf.pdf431.79 kBAdobe PDF    Request a copy



FacebookTwitterDeliciousLinkedInGoogle BookmarksBibTex Format mendeley Endnote DiggMySpace

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.