Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/35437
Title: Diagnostic challenges of single plaque-like lesion paucibacillary leprosy
Authors: Barbieri, Raquel Rodrigues
Sales, Anna Maria
Illarramendi, Ximena
Moraes, Milton Ozório
Nery, José Augusto da Costa
Moreira, Suelen Justo Maria
Sarno, Euzenir Nunes
Machado, Alice de Miranda
Bozza, Fernando Augusto
Affilliation: Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil
Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil.
Abstract: The diagnosis of single-lesion paucibacillary leprosy remains a challenge. Reviews by expert dermatopathologists and quantitative polymerase chain reaction (qPCR) results obtained from 66 single-plaque biopsy samples were compared. Histological findings were graded as high (HP), medium (MP) or low (LP) probability of leprosy or other dermatopathy (OD). Mycobacterium leprae-specific genes were detected using qPCR. The biopsies of 47 out of 57 clinically diagnosed patients who received multidrug therapy were classified as HP/MP, eight of which were qPCR negative. In the LP/OD (n = 19), two out of eight untreated patients showed positive qPCR results. In the absence of typical histopathological features, qPCR may be utilised to aid in final patient diagnosis, thus reducing overtreatment and delay in diagnosis.
Keywords: Paucibacillary leprosy
Diagnosis
Quantitative polymerase chain reaction
Issue Date: 2014
Publisher: Instituto Oswaldo Cruz, Ministério da Saúde
Citation: BARBIERI, Raquel Rodrigues et al. Diagnostic challenges of single plaque-like lesion paucibacillary leprosy. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 109, n. 7, p. 944-947, Nov. 2014.
DOI: 10.1590/0074-0276140212
ISSN: 0074-0276
Copyright: open access
Appears in Collections:IOC - Artigos de Periódicos
INI - Artigos de Periódicos

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