Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/35445
Title: Immunogenicity of synthetic peptide constructs based on PvMSP9E795-A808, a linear B-cell epitope of the P. vivax Merozoite Surface Protein-9
Authors: Silva, Rodrigo Nunes Rodrigues da
Moreira, Daniely Correa
Soares, Isabela Ferreira
Luca, Paula Melo de
Totino, Paulo Renato Rivas
Morgado, Fernanda Nazaré
Henriques, Maria das Graças de Oliveira
Candea, André Luis Peixoto
Singh, Balwan
Galinski, Mary R.
Moreno, Alberto
Ferreira, Joseli Oliveira
Lima Junior, Josué da Costa
Affilliation: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Taxonomia, Bioquímica e Bioprospecção de Fungos. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Farmanguinhos. Laboratório de Farmacologia Aplicada. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Farmanguinhos. Laboratório de Farmacologia Aplicada. Rio de Janeiro, RJ. Brasil.
Emory University. YerkesNational Primate Research Center. Emory Vaccine Center. Atlanta, GA, USA.
Yerkes National Primate Research Center. Emory Vaccine Center. Division of Infectious Diseases. Emory University School of Medicine, Emory University, Atlanta, GA, USA..
Yerkes National Primate Research Center. Emory Vaccine Center. Division of Infectious Diseases. Emory University School of Medicine, Emory University, Atlanta, GA, USA.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ. Brasil.
Abstract: Plasmodium vivax Merozoite Surface Protein-9 (PvMSP-9) is a malaria vaccine candidate naturally immunogenic in humans and able to induce high antibody titers in animals when delivered as a recombinant protein. Recently, we identified the sequence EAAPENAEPVHENA (PvMSP9E795-A808) as the main linear B-cell epitope in naturally exposed individuals. However, the potential of PvMSP9E795-A808 as an immunogen in experimental animal models remained unexplored. Here we assess the immunogenicity of PvMSP9E795-A808 using synthetic peptides. The peptides tested in BALB/c mice include two repeats of the sequence EAAPENAEPVHENA tested alone (peptide RII), or linked to an autologous (PvMSP9 peptide pL; pLRII) or heterologous (p2 tetanus toxin universal T cell epitope; TTRII) T cell epitope. Immune responses were evaluated by ELISA, FLUOROSPOT, and indirect immunofluorescence. We show that all of the peptide constructs tested were immunogenic eliciting specific IgG antibodies at different levels, with a prevalence of IgG1 and IgG2. Animals immunized with synthetic peptides containing T cell epitopes (pLRII or TTRII) had more efficient antibody responses that resulted in higher antibody titers able to recognize the native protein by immunofluorescence. Relevantly, the frequency of IFN-γ secreting SFC elicited by immunization with TTRII synthetic peptide was comparable to that reported to the PvMSP9-Nt recombinant protein. Taken together, our study indicates that PvMSP9E795-A808 is highly immunogenic in mice and further studies to evaluate its value as promising vaccine target are warranted. Moreover, our study supports the critical role of CD4 T cell epitopes to enhance humoral responses induced by subunit based vaccines.
Keywords: Malaria
Linear B-cell epitope
T-cell epitope
Peptide-based immunogens
Immunogenicity
Antigenicity
keywords: Malária
Epítopo linear de células B
Epitopos de Linfócito T
Imunógenos à base de peptídeos
Imunogenicidade
Antigenicidade
DeCS: Epitopos de Linfócito T
Issue Date: 2019
Publisher: Elsevier 12 Months
Citation: SILVA, Rodrigo Nunes Rodrigues da et al. Immunogenicity of synthetic peptide constructs based on PvMSP9E795-A808, a linear B-cell epitope of the P. vivax Merozoite Surface Protein-9. Vaccine, v. 37, p. 306-313, 2019.
DOI: 10.1016/j.vaccine.2018.10.016
ISSN: 0264-410X
Copyright: open access
Appears in Collections:Farmanguinhos - Artigos de Periódicos
IOC - Artigos de Periódicos

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