Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/35760
Title: Domestic and wild mammals infection by Trypanosoma evansi in a pristine area of the Brazilian Pantanal region
Authors: Herrera, Heitor M.
Norek, Aneska
Freitas, Tatiana P. T.
Rademaker, Vitor
Fernandes, Octàvio
Jansen, Ana Maria
Affilliation: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Protozoologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Medicina Tropical. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Protozoologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Medicina Tropical. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Medicina Tropical. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Protozoologia. Rio de Janeiro, RJ, Brasil.
Abstract: The objective of this study was to estimate the Trypanosoma evansi infection rate and epizootical status of wild and domestic animals from the Brazilian Pantanal region using a standardized polymerase chain reaction (PCR). We used a simple DNA extraction method based on Chelex resin (BioRad, USA) on blood eluted from filter paper confetti. Primers directed to repetitive nuclear DNA sequences were used in the PCR, and could detect 30 fg of T. evansi DNA. The analytical specificity of the assay was evaluated using T. evansi, T. rangeli, T. cruzi, Leishmania braziliensis, Crithidia fasciculata and Herpetomonas muscarum DNAs as templates and the technique showed the expected 164 bp specific band solely for Trypanozoon trypanosomes. The application of the standardized PCR protocol in 274 field samples from domestic and wild mammals from the Rio Negro (Brazilian Pantanal region), showed a general infection rate of 10.2% while the traditional parasitological technique (direct search of the protozoan by the microematocrit centrifugue technique) was able to determine infection in only 1.1% of the animals. The peccaries and feral pigs were found to be the animals most frequently infected with T. evansi (24.4% and 30.7%, respectively). Both sampling and extraction methods used herein, showed to be simple and efficient to be applied in epidemiological surveys using PCR.
Keywords: Trypanosoma evansi
Brazilian Pantanal region
Domestic mammals
Wild mammals
Infection
keywords: Trypanosoma evansi
Mamíferos domésticos
Mamíferos selvagens
Pantanal brasileiro
Infecção
Issue Date: 2005
Publisher: Springer Verlag (Germany)
Citation: HERRERA, Heitor M. et al. Domestic and wild mammals infection by Tripanosoma evansi in a pristine area of the Brazilian Pantanal region, Parasitology Research, v. 96, p. 121-126, 2005.
DOI: 10.1007/s00436-005-1334-6
ISSN: 0932-0113
Copyright: restricted access
Appears in Collections:IOC - Artigos de Periódicos

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